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Millipore/HTS004M | ChemiSCREEN™ CXCR4 Membrane Preparation/HTS004M/200 units
  • Millipore/HTS004M | ChemiSCREEN™ CXCR4 Membrane Preparation/HTS004M/200 units

Millipore/HTS004M | ChemiSCREEN™ CXCR4 Membrane Preparation/HTS004M/200 units

价格: ¥7152.00 市场价: 11920.00
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货号: HTS004M
品牌: Millipore
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    • Description
      CatalogueNumberHTS004M
      BrandFamilyChemicon®
      TradeName
      • ChemiScreen
      • Chemicon
      DescriptionChemiSCREEN™CXCR4MembranePreparation
      OverviewFull-lengthhumanCXCR4CDNA
      BackgroundInformationThechemokineSDF-1aanditsGPCRreceptorCXCR4haveaone-to-onespecificitythatisuniqueamongchemokinesandtheirreceptors.SDF-1abindstoCXCR4expressedonhematopoieticandlymphopoieticcells,anddirectstheirtraffickingtoandretentioninhemato-andlymphatopoieticorgansandsitesofinflammation(Kuciaetal.,2004).CXCR4isexpressedonseveraltumorcelllineages,andmightberesponsIBLeformetastasistositesofSDF-1aexpression,suchasboneandlymphnodes(Mulleretal.,2001).Inaddition,CXCR4isacoreceptorfortheHIVenvelopeglycoproteingp120(Fengetal.,1996).SmallmoleculeantagoNISTsofCXCR4havebeendevelopedandshowntoinhibitinfectivityofT-tropicHIVstrainsandtoimpairgrowthofbraintumors(Arakakietal.,1999;Rubinetal.,2003).Chemicon"sCXCR4membranepreparationsarecrudemembranepreparationsmadefromourproprietarystablerecombinantcelllinestoensurehigh-levelofGPCRsurfaceexpression;thus,theyareidealtoolsforscreeningforantagonistsofinteractionsbetweenCXCR4andSDF-1a.RADIolabeledSDF-1abindstotheCXCR4membraneswithaKdof0.9nM.With0.04-0.13nM125I-labeledSDF-1a,anapproximately3-foldwindowofspecificbindingisobtained.
      ProductInformation
      FormatMembranes
      PresentationPackagingbuffer:50mMTrispH7.4,10%glyceroland1%BSA

      Packagingmethod:Membranesproteinwereadjustedto0.5mg/mlin1mlpackagingbuffer,rapidlyfrozen,andstoredat-80°C.
      StorageandShippingInformation
      StorageConditionsMaintainfrozenat-70°Cforupto2years.Donotfreezeandthaw.
      Applications
      ApplicationHumanCXCR4GPCRmembranepreparationforRadioligandbindingAssays>PgammaSbinding.
      KeyApplications
      • Radioligandbindingassay
      • GTPγSbinding
      ApplicationNotesRadioligandbindingassayandGTPgammaSbinding.
      BIOLOGicalInformation
      Concentration2mL,0.5mg/mL
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodesaCXCchemokinereceptorspecificforstromalcell-derivedfactor-1.Theproteinhas7transmembraneregionsandislocatedonthecellsurface.ItactswiththeCD4proteintosupportHIVentryintocellsandisalsohighlyexpressedinbreastcancercells.MutationsinthisgenehavebeenassociatedwithWHIM(warts,hypogammaglobulinemia,infections,andmyelokathexis)syndrome.Alternatetranscriptionalsplicevariants,encodingdifferentisoforms,havebeencharacterized.
      GeneSymbol
      • CXCR4
      • WHIM
      • NPY3R
      • NPYY3R
      • FB22
      • LAP3
      • Fusin
      • CXCR-4
      • LCR1
      • NPYR
      • HM89
      • fusin
      • HSY3RR
      • CXC-R4
      • NPYY3
      • NPYRL
      • D2S201E
      • CD184
      • LESTR
      GPCRClassA
      HostCellsChem-1
      ProteinTargetCXCR4
      TargetSub-FamilyChemokine
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P61073#ReceptorfortheC-X-CchemokineCXCL12/SDF-1.Transducesasignalbyincreasingtheintracellularcalciumionslevel.Involvedinhaematopoiesisandincardiacventricularseptumformation.Playsalsoanessentialroleinvascularizationofthegastrointestinaltract,probablybyregulatingvascularbranchingand/orremodelingprocessesinendothelialcells.Couldbeinvolvedincerebellardevelopment.IntheCNS,couldmediatehippocampal-neuronsurvival.Actsasacoreceptor(CD4beingtheprimaryreceptor)forHIV-1X4isolatesandasaprimaryreceptorforsomeHIV-2isolates.PromotesEnv-mediatedfusionofthevirus.
      SIZE:352aminoacids;39746Da
      SUBUNIT:InteractswithHIV-1surfaceproteingp120andTat.
      SUBCELLULARLOCATION:Cellmembrane;Multi-passmembraneprotein.
      TISSUESPECIFICITY:Expressedinnumeroustissues,suchasperipheralbloodleukocytes,spleen,thymus,spinalcord,heart,placenta,lung,liver,skeletalmuscle,kidney,pancreas,cerebellum,cerebralcortexandmedulla(inmicrogliaaswellasinastrocytes),brainmicrovascular,coronaryarteryandumbilicalcordendothelialcells.Isoform1ispredominantinalltissuestested.
      DOMAIN:SwissProt:P61073Theamino-terminusiscriticalforligandbinding.ResiduesinallfourextracellularregionscontributetoHIV-1coreceptoractivity.
      PTM:Sulfated.
      DISEASE:SwissProt:P61073#DefectsinCXCR4areacauseofWHIMsyndrome[MIM:193670];alsocalledwarts,hypogammaglobulinemia,infections,andmyelokathexis.WHIMsyndromeisanimmunodeficiencydiseasecharacterizedbyneutropenia,hypogammaglobulinemiaandextensivehumanpapillomavirus(HPV)infection.Despitetheperipheralneutropenia,bonemarrowaspiratesfromaffectedindividualscontainabundantmaturemyeloidcells,aconditiontermedmyelokathexis.
      SIMILARITY:BelongstotheG-proteincoupledreceptor1family.
      IncubationConditionsRECOMMENDEDASSAYCONDITIONS:Membranesaremixedwithradioactiveligandandunlabeledcompetitor(seeFigures1and2forconcentrationstested)inbindingbufferinanonbinding96-wellplate,andincubatedfor1-2h.Priortofiltration,aGF/C96-wellfilterplateiscoatedwith0.33%polyethyleneiminefor30min,thenwashedwith50mMHEPES,pH7.4,0.5%BSA.Bindingreactionistransferredtothefilterplate,andwashed3times(1mLperwellperwash)withWashBuffer.Theplateisdriedandcounted.

      Bindingbuffer:50mMHepes,pH7.4,5mMMgCl2,1mMCaCl2,0.2%BSA,filteredandstoredat4°C

      Radioligand:[125I]SDF-1alpha(Perkinelmer#:NEX346)

      WashBuffer:50mMHepes,pH7.4,500mMNaCl,0.1%BSA,filteredandstoredat4°C.

      Onevialcontainsenoughmembranesforatleast200assays(units),whereanunitistheamountofmembranethatwillyieldgreaterthan4-foldsignal:backgroundwith125I-labeledSDF-1alphaat0.13nM.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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