Chickenswereimmunizedwiththe6X-HistidinepeptideconjugatedtokeyholelimpethemocyaninproteinandemulsifiedinFreund’sadjuvants.Aftermultipleinjections,eggswerecollectedfromthehens,IgYfractionswerepreparedfromtheyolks,andthenaffinity-purifiedantibodieswerepreparedusing6X-Histidinepeptideconjugatedtoanagarosematrix.Tomakeupthefinalmixture,theIgYfractionwasspikedwithaffinitypurifiedantibody,mixedwithglycerolandthimerosal,andfilter-sterilized.