Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer:
In 1x PBS, pH 7.4
Storage Instruction:
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
MSDS:
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Interspecies Antigen Sequence:
Mouse (94); Rat (94)
Datasheet:
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of AAK1 expression in transfected 293T cell line by AAK1 monoclonal antibody (M02), clone 4B10.Lane 1: AAK1 transfected lysate(52 KDa).Lane 2: Non-transfected lysate.
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Western Blot (Recombinant protein)
Protocol Download
Immunofluorescence
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Immunofluorescence of monoclonal antibody to AAK1 on HeLa cell . [antibody concentration 10 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged AAK1 is 0.1 ng/ml as a capture antibody.
Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq