Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (75.02 KDa) .
Storage Buffer:
In 1x PBS, pH 7.4
Storage Instruction:
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
MSDS:
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Datasheet:
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Applications
Western Blot (Cell lysate)
TUBA1 monoclonal antibody (M01), clone 2E11. Western Blot analysis of TUBA1 expression in HeLa.
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Western Blot (Cell lysate)
TUBA1 monoclonal antibody (M01), clone 2E11. Western Blot analysis of TUBA1 expression in different Cell lines and Human Tissue.
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Western Blot (Transfected lysate)
Western Blot analysis of TUBA1 expression in transfected 293T cell line by TUBA1 monoclonal antibody (M01), clone 2E11.Lane 1: TUBA1 transfected lysate(49.9 KDa).Lane 2: Non-transfected lysate.
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Western Blot (Recombinant protein)
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Immunofluorescence
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Immunofluorescence of monoclonal antibody to TUBA1 on HeLa cell. [antibody concentration 10 ug/ml]
Immunoperoxidase of monoclonal antibody to TUBA1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]
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ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of TUBA1 over-expressed 293 cell line, cotransfected with TUBA1 Validated Chimera RNAi ( Cat # H00007277-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TUBA1 monoclonal antibody (M01), clone 2E11 (Cat # H00007277-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. [provided by RefSeq