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Abnova/GAPDH monoclonal antibody (M01), clone 3C2/1kit/H00002597-M01
  • Abnova/GAPDH monoclonal antibody (M01), clone 3C2/1kit/H00002597-M01

Abnova/GAPDH monoclonal antibody (M01), clone 3C2/1kit/H00002597-M01

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货号: H00002597-M01
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      • Specification
      • Gene Info
      • Pathway
      • Disease
      • Related Products
      • List All
      • Specification
      • Product Description:
      • Mouse monoclonal antibody raised against a partial recombinant GAPDH.
      • Immunogen:
      • GAPDH (NP_002037, 226 a.a. ~ 335 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
      • Sequence:
      • GKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE
      • Host:
      • Mouse
      • Reactivity:
      • Human, Mouse, Rat
      • Isotype:
      • IgG1 Kappa
      • Quality Control Testing:
      • Antibody Reactive Against Recombinant Protein.QC Testing of H00002597-M01Western Blot detection against Immunogen (37.84 KDa) .
      • Storage Buffer:
      • In 1x PBS, pH 7.4
      • Storage Instruction:
      • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
      • MSDS:
      • PDF DownloadDownload
      • Datasheet:
      • PDF DownloadDownload
      • Publication Reference
      • 1.
      • Rosmarinic Acid Exhibits Broad Anti-Enterovirus A71 Activity by Inhibiting the Interaction between the Five-Fold Axis of Capsid VP1 and Cognate Sulfated Receptors.Chung-Fan Hsieh, Jia-Rong Jheng, Guan-Hua Lin, Yu-Li Chen, Jin-Yuan Ho, Chien-Jou Liu, Kuei-Yang Hsu, Yuan-Siao Chen, Yoke Fun Chan, Hui-Ming Yu, Pei-Wen Hsieh, Jyh-Haur Chern, Jim-Tong Horng.Emerg Microbes Infect. 2020 Dec;9(1):1194-1205. doi: 10.1080/22221751.2020.1767512.
      • 2.
      • Mannose receptor is an HIV restriction factor counteracted by Vpr in macrophages.Lubow J, Virgilio MC, Merlino M, Collins DR, Mashiba M, Peterson BG, Lukic Z, Painter MM, Gomez-Rivera F, Terry V, Zimmerman G, Collins KL.Elife. 2020 Mar 2;9. pii: e51035. doi: 10.7554/eLife.51035.
      • 3.
      • GNS561, a new lysosomotropic small molecule, for the treatment of intrahepatic cholangiocarcinoma.Brun S, Bassissi F, Serdjebi C, Novello M, Tracz J, Autelitano F, Guillemot M, Fabre P, Courcambeck J, Ansaldi C, Raymond E, Halfon P.Invest New Drugs. 2019 Dec;37(6):1135-1145. doi: 10.1007/s10637-019-00741-3. Epub 2019 Feb 19.
      • 4.
      • ISGF3 with reduced phosphorylation is associated with constitutive expression of interferon-induced genes in aging cells.Yamagami M, Otsuka M, Kishikawa T, Sekiba K, Seimiya T, Tanaka E, Suzuki T, Ishibashi R, Ohno M, Koike K.NPJ Aging Mech Dis. 2018 Nov 15;4:11. doi: 10.1038/s41514-018-0030-6. eCollection 2018.
      • 5.
      • A large-scale proteomic identification of targets of cellular miR-197 downregulated by enterovirus A71.Tang WF, Huang RT, Chien KY, Tang P, Horng JT.J Proteome Res. 2018 Oct 29. doi: 10.1021/acs.jproteome.8b00762. [Epub ahead of print]
      • 6.
      • A novel role of ER stress signal transducer ATF6 in regulating enterovirus A71 viral protein stability.Jheng JR, Lau KS, Lan YW, Horng JT.J Biomed Sci. 2018 Jan 31;25(1):9.
      • 7.
      • Inhibition of autolysosome formation in host autophagy by Trypanosoma cruzi infection.Onizuka Y, Takahashi C, Uematsu A, Shinjo S, Seto E, Nakajima-Shimada J.Acta Trop. 2017 Feb 21;170:57-62
      • 8.
      • Radioprotective effects of genistein on HL-7702 cells via the inhibition of apoptosis and DNA damage.Song L, Ma L, Cong F, Shen X, Jing P, Ying X, Zhou H, Jiang J, Yan H.Cancer Lett. 2015 Jun 18. [Epub ahead of print]
      • 9.
      • Supplementation of strontium to a chondrogenic medium promotes chondrogenic differentiation of human dedifferentiated fat cells.Okita N, Honda Y, Kishimoto N, Liao W, Azumi E, Hashimoto Y, Matsumoto NTissue Eng Part A. 2015 Feb 11.
      • 10.
      • RacGTPase-activating protein 1 interacts with hepatitis C virus polymerase NS5B to regulate viral replication.Wu MJ, Ke PY, Horng JTBiochem Biophys Res Commun. 2014 Oct 8. pii: S0006-291X(14)01794-X. doi: 10.1016/j.bbrc.2014.10.008.
      • 11.
      • miR-106b is overexpressed in medulloblastomas and interacts directly with PTEN.Li KK, Xia T, Ma FM, Zhang R, Mao Y, Wang Y, Zhou L, Lau KM, Ng HK.Neuropathol Appl Neurobiol. 2014 Jul 8. doi: 10.1111/nan.12169.
      • 12.
      • Reticulon 3 interacts with NS4B of the hepatitis C virus and negatively regulates viral replication by disrupting NS4B self-interaction.Wu MJ, Ke PY, Hsu JT, Yeh CT, Horng JTCell Microbiol. 2014 Jun 4. doi: 10.1111/cmi.12318.
      • 13.
      • Receptor for Activated Protein Kinase C: Requirement for Efficient MicroRNA Function and Reduced Expression in Hepatocellular Carcinoma.Otsuka M, Takata A, Yoshikawa T, Kojima K, Kishikawa T, Shibata C, Takekawa M, Yoshida H, Omata M, Koike K.PLoS One. 2011;6(9):e24359. Epub 2011 Sep 15.
      • 14.
      • Expression and roles of Slit/Robo in human ovarian cancer.Dai CF, Jiang YZ, Li Y, Wang K, Liu PS, Patankar MS, Zheng J.Histochem Cell Biol. 2011 Apr 5. [Epub ahead of print]
      • 15.
      • Anti-angiogenic effect of triterpenoidal saponins from Polygala senega.Arai M, Hayashi A, Sobou M, Ishida S, Kawachi T, Kotoku N, Kobayashi M.J Nat Med. 2010 Nov 2. [Epub ahead of print]
      • 16.
      • The Effect of Allelic Variation in Aldo-Keto Reductase 1C2 on the In Vitro Metabolism of Dihydrotestosterone.Takahashi RH, Grigliatti TA, Reid RE, Riggs KW.J Pharmacol Exp Ther. 2009 Jun;329(3):1032-9. Epub 2009 Mar 3.
      • Applications
      • Western Blot (Cell lysate)
      • Western Blot (Cell lysate)
      • GAPDH monoclonal antibody (M01), clone 3C2. Western Blot analysis of GAPDH expression in HeLa ( Cat # L013V1 ).
      • PDF DownloadProtocol Download
      • Western Blot (Cell lysate)
      • Western Blot (Cell lysate)
      • GAPDH monoclonal antibody (M01), clone 3C2. Western Blot analysis of GAPDH expression in PC-12(Cat # L012V1 ).
      • PDF DownloadProtocol Download
      • Western Blot (Cell lysate)
      • Western Blot (Cell lysate)
      • GAPDH monoclonal antibody (M01), clone 3C2. Western Blot analysis of GAPDH expression in Raw 264.7(Cat # L024V1 ).
      • PDF DownloadProtocol Download
      • Western Blot (Cell lysate)
      • Western Blot (Cell lysate)
      • GAPDH monoclonal antibody (M01), clone 3C2 Western Blot analysis of GAPDH expression in A-431 ( Cat # L015V1 ).
      • PDF DownloadProtocol Download
      • Western Blot (Cell lysate)
      • Western Blot (Cell lysate)
      • GAPDH monoclonal antibody (M01), clone 3C2. Western Blot analysis of GAPDH expression in NIH/3T3(Cat # L018V1 ).
      • PDF DownloadProtocol Download
      • Western Blot (Transfected lysate)
      • Western Blot (Transfected lysate)
      • Western Blot analysis of GAPDH expression in transfected 293T cell line by GAPDH monoclonal antibody (M01), clone 3C2.Lane 1: GAPDH transfected lysate(36.1 KDa).Lane 2: Non-transfected lysate.
      • PDF DownloadProtocol Download
      • Western Blot (Recombinant protein)
      • PDF DownloadProtocol Download
      • Sandwich ELISA (Recombinant protein)
      • Sandwich ELISA (Recombinant protein)
      • Detection limit for recombinant GST tagged GAPDH is 0.03 ng/ml as a capture antibody.
      • PDF DownloadProtocol Download
      • ELISA
      • Application Image
      • Western Blot (Cell lysate)
      • Western Blot (Cell lysate)
      • enlarge
      • Western Blot (Cell lysate)
      • Western Blot (Cell lysate)
      • enlarge
      • Western Blot (Cell lysate)
      • Western Blot (Cell lysate)
      • enlarge
      • Western Blot (Cell lysate)
      • Western Blot (Cell lysate)
      • enlarge
      • Western Blot (Cell lysate)
      • Western Blot (Cell lysate)
      • enlarge
      • Western Blot (Transfected lysate)
      • Western Blot (Transfected lysate)
      • enlarge
      • Western Blot (Recombinant protein)
      • Sandwich ELISA (Recombinant protein)
      • Sandwich ELISA (Recombinant protein)
      • enlarge
      • ELISA
      • Gene Information
      • Entrez GeneID:
      • 2597
      • GeneBank Accession#:
      • NM_002046
      • Protein Accession#:
      • NP_002037
      • Gene Name:
      • GAPDH
      • Gene Alias:
      • G3PD,GAPD,MGC88685
      • Gene Description:
      • glyceraldehyde-3-phosphate dehydrogenase
      • Omim ID:
      • 138400
      • Gene Ontology:
      • Hyperlink
      • Gene Summary:
      • The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq
      • Other Designations:
      • OTTHUMP00000174431,OTTHUMP00000174432,aging-associated gene 9 protein,glyceraldehyde 3-phosphate dehydrogenase
      • Gene Pathway
      • Alzheimer"s disease
      • Biosynthesis of alkaloids derived from histidine and purine
      • Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
      • Biosynthesis of alkaloids derived from shikimate pathway
      • Biosynthesis of alkaloids derived from terpenoid and polyketide
      • Biosynthesis of phenylpropanoids
      • Biosynthesis of plant hormones
      • Biosynthesis of terpenoids and steroids
      • Glycolysis / Gluconeogenesis
      • Metabolic pathways
      • Related Disease
      • Alzheimer Disease
      • Alzheimer disease
      • Cardiovascular Diseases
      • Diabetes Complications
      • Metabolic Syndrome X
      • Neoplasms
      • Nerve Degeneration
      • Osteoporosis
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