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Millipore/GF094 | Vascular Endothelial Growth Factor, Recombinant human, 165aa isoform/GF094/10 µg
  • Millipore/GF094 | Vascular Endothelial Growth Factor, Recombinant human, 165aa isoform/GF094/10 µg

Millipore/GF094 | Vascular Endothelial Growth Factor, Recombinant human, 165aa isoform/GF094/10 µg

价格: ¥3384.00 市场价: 5640.00

货号: GF094
品牌: Millipore
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    • Description
      CatalogueNumberGF094
      ReplacesGF025
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionVascularEndothelialGrowthFactor,Recombinanthuman,165aaisoform
      OverviewVascularEndothelialCellGrowthFactor(VEGF)isasecretedhomodimericproteinsecretedbyavarietyofvascularizedtissues.ThereportedactivitiesofVEGFincludestimulationofendothelialcellgrowth,angiogenesis,andcapillarypermeABIlity.HumanVEGFisa38.2kDahomodimericproteinconsistingoftwo165aminoacidpolypeptidechains.
      AlternateNames
      • VEGF
      ProductInformation
      PresentationLyophilizedfromsterilefiltered20mMaceticacid,containingnoadditives.
      StorageandShippingInformation
      StorageConditionsThelyophilizedpowderisstableforafewweeksatroomtemperature,butbeststoredat-20°Cforuptooneyearfromdateofreceipt.Afteraquickspin,reconstitutewithsterilewatertoaconcentrationof0.1-1.0mg/mL.Reconstitutedmaterialcanbestoredat2°C-8°Cforoneweek,butundilutedaliquotsat-20°Carerecommended.Avoidrepeatedfreeze/thawcycles.
      Applications
      ApplicationVascularEndothelialCellGrowthFactor(VEGF)isasecretedhomodimericproteinsecretedbyavarietyofvascularizedtissues.
      KeyApplications
      • CellCulture
      ApplicationNotesFormostinvitroapplications,humanVEGFexertsitsBIOLOGicalactivityintheconcentrationrangeof0.1to50.0ng/mL.Respondingcellsare(partiallist):Endothelialcells.
      BiologicalInformation
      PurityGreaterthan98%bySDS-PAGE.Endotoxinlevelislessthan0.1ngperμg(1EU/ug)ofVEGF
      SourceE.coli
      SpecificActivityThebiologicalactivityofMillipore'shumanVEGFwasdeterminedbyitsmitogenicactivityonhumanumbilicalveinendothelialcells(HUVEC).Mitogenicactivitywasobtainedinaconcentrationrangeof1.0-8.0ng/mL.
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneisamemberofthePDGF/VEGFgrowthfactorfamilyandencodesaproteinthatisoftenfoundasadisulfidelinkedhomodimer.Thisproteinisaglycosylatedmitogenthatspecificallyactsonendothelialcellsandhasvariouseffects,includingmediatingincreasedvascularpermeability,inducingangiogenesis,vasculogenesisandendothelialcellgrowth,promotingcellmigration,andinhibitingapoptosis.ElevatedlevelsofthisproteinislinkedtoPOEMSsyndrome,alsoknownasCrow-Fukasesyndrome.Mutationsinthisgenehavebeenassociatedwithproliferativeandnonproliferativediabeticretinopathy.Alternativelysplicedtranscriptvariants,encodingeitherfreelysecretedorcell-associatedisoforms,havebeencharacterized.Thereisalsoevidencefortheuseofnon-AUG(CUG)translationinitiationsitesupstreamof,andin-framewiththefirstAUG,leADIngtoadditionalisoforms.
      GeneSymbol
      • VEGFA
      • MGC70609
      • VPF
      • VEGF
      • VEGF-A
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P15692#Growthfactoractiveinangiogenesis,vasculogenesisandendothelialcellgrowth.Inducesendothelialcellproliferation,promotescellmigration,inhibitsapoptosis,andinducespermeabilizationofbloodvessels.BindstotheVEGFR1/Flt-1andVEGFR2/Kdrreceptors,heparansulfateandheparin.Neuropilin-1bindsisoformsVEGF-165andVEGF-145.
      SIZE:232aminoacids;27042Da
      SUBUNIT:Homodimer;disulfide-linked.AlsofoundasheterodimerwithPlGF(Bysimilarity).
      SUBCELLULARLOCATION:Secreted.Note=VEGF121isacidicandfreelysecreted.VEGF165ismorebasic,hasheparin-bindingpropertiesand,althoughasignicantproportionremainscell-associated,mostisfreelysecreted.VEGF189isverybasic,itiscell-associatedaftersecretionandisboundavidlybyheparinandtheextracellularmatrix,althoughitmaybereleasedasasolubleformbyheparin,heparinaseorplasmin.
      TISSUESPECIFICITY:TheVEGF189,VEGF-165andVEGF-121isoformsarewidelyexpressed,whereastheVEGF206andVEGF-145areuncommon.
      SIMILARITY:SwissProt:P15692##BelongstothePDGF/VEGFgrowthfactorfamily.
      StemCellType
      • NeuralStemCells
      • MesenchymalStemCells
      • HematopoieticStemCells
      MolecularWeight38.2kDa
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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