Properties:
RelatedCategories | Analytical/Chromatography,GEHealthcareLifeSciencesProteinSamplePreparationFormats,HiTrap&PD-10Cartridges,HiTrapSystem/syringe,MolecularBIOLOGy, |
shelflife | Pleasebeawarethisproductmaybeshipped90daysbeforetheexpirationdate.Formoreinformationonthebatchspecificexpirationdate,pleasecontacttechnicalservice. |
packaging | packof5×5 mL |
mfr.no. | GEHealthcare,17-0921-04 |
parameter | 42psi |
bedvolume | 5 mL |
columnI.D. | 16 mm |
matrix | 6%cross-linkedagarose |
particlesize | 45-165 μm |
averagediameter | 90 μm |
cleaning | 2-14(Mediumunchargedwithmetalions.) |
workingrange | 3-12 |
capacity | ~40 mgbindingcapacity(histidine-taggedprotein/mlmediumwhenchangedwithNi2+)(Protein-andmetal-iondependent.) |
suitABIlity | suitableforbioprocessmedium |
Description:
AnalysisNote
www.geLifesciences.com.
LegalInformation
IMACSepharoseproducts,NiSepharoseproductsandFeSepharoseproducts
TheseproductsaresoldunderalicensefromSigma-AldrichunderpatentnumberEP1276716(Metalchelatingcompositions)andequivalentpatentsandpatentapplicationsinothercountries.
HiPrepisatrademarkofGEHealthcare
HiScreenisatrademarkofGEHealthcare
HiTrapisaregisteredtrademarkofGEHealthcare
SepharoseisaregisteredtrademarkofGEHealthcare
Application
IMACSepharose®FFissuppliedfreeofmetalions.Itischargedbytheuserwiththetransitionmetalionofchoice(e.g.Cu2+,Zn2+,Ni2+,orCo2+);thesemetalionswillbindtothecovalentlyimmobilizedchelatingligandontheSepharose.
IMACSepharose®6FastFLowconsistsof90?mbeadsofhighlycross-linkedAgarosetowhichachelatinggrouphasbeencovalentlycoupled.Thischelatinggroupcanbechargedwithsuitablemetalions,specificforyourtargetProteinofinterest.ThebeadsizeofIMACSepharose®6FastFLowenableshighflowproperties,makingthemediumanexcellentchoiceforscalinguppurificationatlaboratoryscaleandpurificationofhistidine-taggedProteinsatprocessscale.LowmetalionleakagemeansthattheactivityofthepurifiedProteinisretainedandtheriskofprecipitationreduced,whichresultsinincreasedpurity,activity,andyieldofthetargetProtein.LeakageofmetalionsintotheelutedtargetProteinpoolfromIMACSepharose®6FastFLowisgenerallyLowundernormalconditions.
IMACSepharose®6FastFLowishighlystableandcompatIBLewithawiderangeofcommonadditives.Thishelpsmaintainbiologicalactivityandincreaseproductyield,whileatthesametimegreatlyexpandingtherangeofsuitableoperatingconditions.
ThemediumisavailableprepackedinHiTrap®HiScreen™andHiPrep™columnformatsforconvenientpurificationofhistidine-taggedProteinsatlaboratoryscale.Initialpurificationusinga1mLHiTrap®IMACFFcolumncanbescaledupfurtherwiththe5mLcolumn.Purificationcanbescaledupevenmoreusing20mLHiPrep™IMACFF16/10columns.PrepackedHiScreen™IMACFFwitha10cmbedheightistheformatofchoiceformethodoptimizationandprocessdevelopment.
FeaturesandBenefits
• Foroptimizingpurificationofhistidine-taggedProteinswhenNi2+isnotthebestchoiceofmetalion.
• Convenientlychargewithyourmetalofchoice.
• Optimalbeadsizeforscalingup.
• Highbindingcapacity.
Generaldescription
HiTrap®IMACFFisprepackedwithIMACSepharose®6FastFlow.ThemediumischargedwiththemetalionofyourchoiceforImmobilizedMetalionAffinityChromatography(IMAC)andsubsequentpurificationofpolyhistidinetaggedproteins.
Safety:
Symbol
GHS02
Precautionarystatements
P210-P370+P378
RIDADR
UN1170-class3-PG3-Ethanol,solution