Labeled FISH probes for identification of gene translocation using Fluoresecent In Situ Hybridization Technique. (Technology)
Quality Control Testing:
Representative images of normal human cell (lymphocyte) stain with the dual color FISH probe. The left image is chromosomes at metaphase, and the right image is an interphase nucleus.
Storage Instruction:
Store at 4°C in the dark.
Note:
Hybridization position of the probes on the chromosome.
Probe 1:Size:Fluorophore:Location:
IGHApproximately 1550kbFITC14q32
Probe 2:Size:Fluorophore:Location:
BCL10Approximately 610kbTexas Red1p22
Origin:
Human
Source:
Genomic DNA
Notice:
We strongly recommend the customer to use FFPE FISH PreTreatment Kit 1 (Catalog #: KA2375 or KA2691) for the pretreatment of Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections.
Regulatory Status:
For research use only (RUO)
Datasheet:
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Applications
Fluorescent In Situ Hybridization (Cell)
Protocol Download
Application Image
Fluorescent In Situ Hybridization (Cell)
IGH
BCL10
Gene Information
Entrez GeneID:
3492
Gene Name:
IGH
Gene Alias:
IGH,IGH.1@,IGHDY1,MGC72071,MGC88774
Gene Description:
immunoglobulin heavy locus
Gene Ontology:
Hyperlink
Gene Summary:
Immunoglobulins recognize foreign antigens and initiate immune responses such as phagocytosis and the complement system. Each immunoglobulin molecule consists of two identical heavy chains and two identical light chains. This region represents the germline organization of the heavy chain locus. The locus includes V (variable), D (diversity), J (joining), and C (constant) segments. During B cell development, a recombination event at the DNA level joins a single D segment with a J segment; this partially rearranged D-J gene is then joined to a V segment. The rearranged V-D-J is then transcribed with the IGHM constant region; this transcript encodes a mu heavy chain. Later in development B cells generate V-D-J-Cmu-Cdelta pre-messenger RNA, which is alternatively spliced to encode either a mu or a delta heavy chain. Mature B cells in the lymph nodes undergo switch recombination, so that the V-D-J gene is brought in proximity to one of the IGHG, IGHA, or IGHE genes and each cell expresses either the gamma, alpha, or epsilon heavy chain. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase, and by somatic hypermutation, which occurs during B cell maturation in the spleen and lymph nodes. Several V, D, J, and C segments are known to be incapable of encoding a protein and are considered pseudogenes. [provided by RefSeq
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