Description: | DNAzol BD Reagent is a reagent specifically formulated for the isolation of genomic DNA from whole blood. The DNAzol BD Reagent is based on the use of a novel guanidine- detergent lysing solution which hydrolyzes RNA and allows the selective precipitation of DNA from the lysate. The isolation of genomic DNA from blood using DNAzol BD Reagent is fast, efficient and economical. In addition to the isolation of genomic DNA, DNAzol BD Reagent can also be used for the isolation of apoptotic fragments from whole blood and viral DNA from serum. Blood samples are mixed with DNAzol BD Reagent and DNA is precipitated from the resulting lysate with isopropanol. The DNA pellet is washed successively with DNAzol BD Reagent and ethanol, and solubilized. The entire procedure can be completed in about 30 min and the isolated DNA can be used for Southern analysis, dot blot hybridization, molecular cloning, PCR and other molecular biology and biotechnology applications. |