mutaFISH™ CK20wt RNA Probes is designed to detect human CK20 gene on single strand RNA in cells using padlock probe and in situ rolling-circle amplification technology.
Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
We recommend mutaFISH™ RNA Accessory Kit (Catalog #: KA4915) which provides necessary reagents and enzymes for in situ reverse transcription, RNA digestion, mutaFISH™ hybridization, ligation and amplication prior to mutaFISH™.
Protocol:
Protocol Download
Technology:
mutaFISH™ (mutation-specific Fluorescence In Situ Hybridization)
mutation specific, Fluorescence In Situ Hybridization (Cells)
mutaFISH™ staining was performed in situ in CK20 transfected 293T cells. CK20 gene was detected via green signal (FITC).
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mutation specific, Fluorescence In Situ Hybridization (Cells)
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Gene Information
Entrez GeneID:
54474
Gene Name:
KRT20
Gene Alias:
CD20,CK20,K20,KRT21,MGC35423
Gene Description:
keratin 20
Omim ID:
608218
Gene Ontology:
Hyperlink
Gene Summary:
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq
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