mutaFISH™ CK7wt RNA Probes is designed to detect human CK7 gene on single strand RNA in cells using padlock probe and in situ rolling-circle amplification technology.
Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
We recommend mutaFISH™ RNA Accessory Kit (Catalog #: KA4915) which provides necessary reagents and enzymes for in situ reverse transcription, RNA digestion, mutaFISH™ hybridization, ligation and amplication prior to mutaFISH™.
Protocol:
Protocol Download
Technology:
mutaFISH™ (mutation-specific Fluorescence In Situ Hybridization)
mutation specific, Fluorescence In Situ Hybridization (Cells)
mutaFISH™ staining was performed in situ in human HeLa cells. CK7 gene was detected via green signal (FITC).
mutation specific, Fluorescence In Situ Hybridization (FFPE Tissue)
mutaFISH™ staining was performed in situ in mouse FFPE CK7 HeLa tissue. CK7 gene was detected via green signal (FITC).
Application Image
mutation specific, Fluorescence In Situ Hybridization (Cells)
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mutation specific, Fluorescence In Situ Hybridization (FFPE Tissue)
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Gene Information
Entrez GeneID:
3855
Gene Name:
KRT7
Gene Alias:
CK7,K2C7,K7,MGC129731,MGC3625,SCL
Gene Description:
keratin 7
Omim ID:
148059
Gene Ontology:
Hyperlink
Gene Summary:
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq
Other Designations:
cytokeratin 7,keratin, 55K type II cytoskeletal,keratin, simple epithelial type I, K7,keratin, type II cytoskeletal 7,sarcolectin,type II mesothelial keratin K7
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