mutaFISH™ ERCC1wt RNA Probes is designed to detect human ERCC1 gene on single strand RNA in cells using padlock probe and in situ rolling-circle amplification technology.
Supplied Product:
Content:1. RT ERCC1 Primer2. mutaFISH™ ERCC1wt RNA Probe3. Detection Probe-Texas Red X
Storage Instruction:
Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
We recommend mutaFISH™ RNA Accessory Kit (Catalog #: KA4915) which provides necessary reagents and enzymes for in situ reverse transcription, RNA digestion, mutaFISH™ hybridization, ligation and amplication prior to mutaFISH™.
Protocol:
Protocol Download
Technology:
mutaFISH™ (mutation-specific Fluorescence In Situ Hybridization)
Comparison:
FISH Probes vs mutaFISH™ Probes
Fluorophore:
Texas Red X (Excitation Peak (nm): 595; Emission Peak613)
Probe Position:
Regulatory Status:
For research use only (RUO)
Video:
Datasheet:
Download
Applications
mutation specific, Fluorescence In Situ Hybridization (Frozen Tissue)
mutaFISH™ staining was performed in situ in PC-9 cells. ERCC1 gene was detected via red signal (Cy5).
Application Image
mutation specific, Fluorescence In Situ Hybridization (Frozen Tissue)
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Protocol Download
