mutaFISH™ c-Mycwt RNA Probes is designed to detect human c-Myc gene on single strand RNA in cells using padlock probe and in situ rolling-circle amplification technology.
Supplied Product:
Content:1. RT c-Myc Primer2. mutaFISH™ c-Mycwt RNA Probe3. Detection Probe-Texas Red X
Storage Instruction:
Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
We recommend mutaFISH™ RNA Accessory Kit (Catalog #: KA4915) which provides necessary reagents and enzymes for in situ reverse transcription, RNA digestion, mutaFISH™ hybridization, ligation and amplication prior to mutaFISH™.
Protocol:
Protocol Download
Technology:
mutaFISH™ (mutation-specific Fluorescence In Situ Hybridization)
Comparison:
FISH Probes vs mutaFISH™ Probes
Fluorophore:
Texas Red X (Excitation Peak (nm): 595; Emission Peak613)
Probe Position:
Regulatory Status:
For research use only (RUO)
Video:
Datasheet:
Download
Applications
mutation specific, Fluorescence In Situ Hybridization (Cells)
mutaFISH™ staining was performed in situ in human H1975 cells. c-Myc was detected via red signal (Cy5).
Application Image
mutation specific, Fluorescence In Situ Hybridization (Cells)
The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt"s lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq
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