mutaFISH™ EML4wt ALKwt RNA Probes is designed to detect human EML4 and ALK gene rearrangement on single strand RNA in cells using padlock probe and in situ rolling-circle amplification technology.
Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
We recommend mutaFISH™ RNA Accessory Kit (Catalog #: KA4915) which provides necessary reagents and enzymes for in situ reverse transcription, RNA digestion, mutaFISH™ hybridization, ligation and amplication prior to mutaFISH™.
Protocol:
Protocol Download
Technology:
mutaFISH™ (mutation-specific Fluorescence In Situ Hybridization)
Comparison:
FISH Probes vs mutaFISH™ Probes
Fluorophore:
6-HEX (Excitation Peak (nm): 533; Emission Peak (nm): 559)Texas Red X (Excitation Peak (nm): 595; Emission Peak613)
Probe Position:
Regulatory Status:
For research use only (RUO)
Video:
Datasheet:
Download
Applications
mutation specific, Fluorescence In Situ Hybridization (Cells)
mutaFISH™ staining was performed in situ in human H1975 cells. EML4 was detected via purple signal (Texas Red); ALK was not detected.
Application Image
mutation specific, Fluorescence In Situ Hybridization (Cells)
enlarge
ALK
EML4
Gene Information
Entrez GeneID:
238
Gene Name:
ALK
Gene Alias:
CD246,Ki-1,TFG/ALK
Gene Description:
anaplastic lymphoma receptor tyrosine kinase
Omim ID:
105590
Gene Ontology:
Hyperlink
Gene Summary:
The 2;5 chromosomal translocation is frequently associated with anaplastic large cell lymphomas (ALCLs). The translocation creates a fusion gene consisting of the ALK (anaplastic lymphoma kinase) gene and the nucleophosmin (NPM) gene: the 3" half of ALK, derived from chromosome 2, is fused to the 5" portion of NPM from chromosome 5. A recent study shows that the product of the NPM-ALK fusion gene is oncogenic. The deduced amino acid sequences reveal that ALK is a novel receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain. These sequences are absent in the product of the transforming NPM-ALK gene. ALK shows the greatest sequence similarity to LTK (leukocyte tyrosine kinase). ALK plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. [provided by RefSeq
购物车为空，快去购物吧！
4000-520-616
咨询顾问
特别 提示
Protocol Download
