mutaFISH™ BRAF V600E V600wt RNA Probes is designed to detect human BRAF V600E gene mutation on single strand RNA in cells using padlock probe and in situ rolling-circle amplification technology.
Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
We recommend mutaFISH™ RNA Accessory Kit (Catalog #: KA4915) which provides necessary reagents and enzymes for in situ reverse transcription, RNA digestion, mutaFISH™ hybridization, ligation and amplication prior to mutaFISH™.
Protocol:
Protocol Download
Technology:
mutaFISH™ (mutation-specific Fluorescence In Situ Hybridization)
Comparison:
FISH Probes vs mutaFISH™ Probes
Fluorophore:
Aqua 431 (Excitation Peak (nm): 431; Emission Peak (nm): 480)Texas Red X (Excitation Peak (nm): 595; Emission Peak613)
Probe Position:
Regulatory Status:
For research use only (RUO)
Video:
Datasheet:
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Applications
mutation specific, Fluorescence In Situ Hybridization (Cells)
mutaFISH™ staining was performed in situ in human Sk-HEP1 cells. BRAF V600E point mutation was detected via orange signal (Cy3), BRAF V600wt was detected via red signal (Cy5).
mutation specific, Fluorescence In Situ Hybridization (FFPE Tissue)
mutaFISH™ staining was performed in situ in mouse FFPE BRAF 293T tissue. BRAF V600wt was detected via red signal (Texas Red X).
Application Image
mutation specific, Fluorescence In Situ Hybridization (Cells)
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mutation specific, Fluorescence In Situ Hybridization (FFPE Tissue)
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Gene Information
Entrez GeneID:
673
Gene Name:
BRAF
Gene Alias:
B-RAF1,BRAF1,FLJ95109,MGC126806,MGC138284,RAFB1
Gene Description:
v-raf murine sarcoma viral oncogene homolog B1
Omim ID:
115150, 164757, 211980
Gene Ontology:
Hyperlink
Gene Summary:
This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq
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