mutaFISH™ CEP1wt CEP7wt CEP8wt CEP17wt DNA Probes is designed to identify human CEP1, CEP7, CEP8, CEP17 amplification on dsDNA in cells using padlock probe and in situ rolling-circle amplification technology.
Supplied Product:
Content:1. mutaFISH™ CEP1wt DNA Probe2. mutaFISH™ CEP7wt DNA Probe3. mutaFISH™ CEP8wt DNA Probe4. mutaFISH™ CEP17wt DNA Probe5. Detection Probe-FITC6. Detection Probe-6-HEX7. Detection Probe-Texas Red X8. Detection Probe-Aqua 431
Storage Instruction:
Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
We recommend mutaFISH™ DNA Accessory Kit 2 for Cells (Catalog #: KA4928) which provides necessary reagents and enzymes for in situ restriction digestion, exonucleolysis, mutaFISH™ hybridization, ligation and amplification prior to mutaFISH™.
Protocol:
Protocol Download
Technology:
mutaFISH™ (mutation-specific Fluorescence In Situ Hybridization)
mutation specific, Fluorescence In Situ Hybridization (Cells)
mutaFISH™ staining was performed in situ in human PBMC cells. CEP7 was detected via purple signal (Texas Red, recolored), CEP8 was detected via red signal (Cy5) and CEP17 was detected via orange signal (Cy3).
Application Image
mutation specific, Fluorescence In Situ Hybridization (Cells)