mutaFISH™ CEP17wt DNA Probes is designed to identify human CEP17 amplification on dsDNA in cells using padlock probe and in situ rolling-circle amplification technology.
Supplied Product:
Content:1. mutaFISH™ CEP17wt DNA Probe2. Detection Probe-Aqua 431
Storage Instruction:
Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
We recommend mutaFISH™ DNA Accessory Kit 2 for Cells (Catalog #: KA4928) which provides necessary reagents and enzymes for in situ restriction digestion, exonucleolysis, mutaFISH™ hybridization, ligation and amplification prior to mutaFISH™.
Protocol:
Protocol Download
Technology:
mutaFISH™ (mutation-specific Fluorescence In Situ Hybridization)
Comparison:
FISH Probes vs mutaFISH™ Probes
Fluorophore:
Aqua 431 (Excitation Peak (nm): 431; Emission Peak (nm): 480)
Probe Position:
Regulatory Status:
For research use only (RUO)
Video:
Datasheet:
Download
Applications
mutation specific, Fluorescence In Situ Hybridization (Cells)
mutaFISH™ staining was performed in situ in human PBMC cells. CEP17 was detected via orange signal (Cy3).
Application Image
mutation specific, Fluorescence In Situ Hybridization (Cells)
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特别 提示
Protocol Download
