mutaFISH™ AR-V7 ARwt RNA Probes is designed to detect AR wildtype and AR-V7 on single strand RNA in cells using padlock probe and in situ rolling-circle amplification technology.
Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Note:
We recommend mutaFISH™ RNA Accessory Kit (Catalog #: KA4915) which provides necessary reagents and enzymes for in situ reverse transcription, RNA digestion, mutaFISH™ hybridization, ligation and amplication prior to mutaFISH™.
Protocol:
Protocol Download
Technology:
mutaFISH™ (mutation-specific Fluorescence In Situ Hybridization)
Comparison:
FISH Probes vs mutaFISH™ Probes
Fluorophore:
Aqua 431 (Excitation Peak (nm): 431; Emission Peak (nm): 480)Texas Red X (Excitation Peak (nm): 595; Emission Peak613)
Probe Position:
Regulatory Status:
For research use only (RUO)
Video:
Datasheet:
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Applications
mutation specific, Fluorescence In Situ Hybridization (Cells)
Fig.1 mutaFISH™ staining was performed in situ in human CWR22Rv1 cells captured by CytoQuest™ CR. AR-V7 was detected via orange signal (Cy3), and ARwt was detected via red signal (Cy5).Fig.2 mutaFISH™ staining was performed in situ in human PBMC cells. Both AR-V7 and ARwt were not detected.
mutation specific, Fluorescence In Situ Hybridization (Frozen Tissue)
mutaFISH™ staining was performed in situ in human frozen tissue from breast cancer. ARwt was detected via red signal (Cy5), and AR-V7 was detected via orange signal (Cy3).
Application Image
mutation specific, Fluorescence In Situ Hybridization (Cells)
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mutation specific, Fluorescence In Situ Hybridization (Frozen Tissue)
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Gene Information
Entrez GeneID:
367
Gene Name:
AR
Gene Alias:
AIS,DHTR,HUMARA,KD,NR3C4,SBMA,SMAX1,TFM
Gene Description:
androgen receptor
Omim ID:
176807, 300068, 300633, 313200, 313700
Gene Ontology:
Hyperlink
Gene Summary:
The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq
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