Labeled FISH probes for identification of gene amplification using Fluoresecent In Situ Hybridization Technique. (Technology)
Form:
Liquid
Quality Control Testing:
Representative images of normal human cell (lymphocyte) stain with the dual color FISH probe. The left image is chromosomes at metaphase, and the right image is an interphase nucleus.
Supplied Product:
DAPI Counterstain (1500 ng/mL ) 125 uL for each 100 uL FISH Probe
Storage Instruction:
Store at 4°C in the dark.
Note:
Hybridization position of the probes on the chromosome.
Probe 1:Size:Fluorophore:Location:
1p36.33Approximately600kbTexas Red1p36.33
Probe 2:Size:Fluorophore:Location:
CEN1pApproximately780kbFITC1p13.3
Probe Gap:
The gap between two probes is approximately 107,700 kb.
Origin:
Human
Source:
Genomic DNA
Notice:
We strongly recommend the customer to use FFPE FISH PreTreatment Kit 1 (Catalog #: KA2375 or KA2691) for the pretreatment of Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections.
Regulation Status:
For research use only (RUO)
Datasheet:
Download
Publication Reference
1.
Genomic gains and losses in malignant mesothelioma demonstrated by FISH analysis of paraffin-embedded tissues.Takeda M, Kasai T, Enomoto Y, Takano M, Morita K, Kadota E, Iizuka N, Maruyama H, Nonomura A.J Clin Pathol. 2012 Jan;65(1):77-82. Epub 2011 Nov 12.
Applications
Fluorescent In Situ Hybridization (Cell)
Protocol Download
Fluorescent In Situ Hybridization (Formalin/PFA-fixed paraffin-embedded sections)
enlarge this image
Human colon cancer (FFPE) stained with 1p36.33/CEN1p FISH Probe. Human colon cancer showed no 1p36.33 gene amplification
Protocol Download
Application Image
Fluorescent In Situ Hybridization (Cell)
Fluorescent In Situ Hybridization (Formalin/PFA-fixed paraffin-embedded sections)