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Millipore/FCCS100182 | FlowCellect™ Histone H2A.X Phosphorylation Assay Kit/FCCS100182/100 Tests / Kit
  • Millipore/FCCS100182 | FlowCellect™ Histone H2A.X Phosphorylation Assay Kit/FCCS100182/100 Tests / Kit

Millipore/FCCS100182 | FlowCellect™ Histone H2A.X Phosphorylation Assay Kit/FCCS100182/100 Tests / Kit

价格: ¥6240.00 市场价: 10400.00

货号: FCCS100182
品牌: Millipore
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    • Description
      CatalogueNumberFCCS100182
      TradeName
      • FlowCellect
      DescriptionFlowCellect™HistoneH2A.XPhosphorylationAssayKit
      OverviewMillipore’sFlowCellect™HistoneH2A.XPhosphorylationAssaykitincludesonedirectlyconjugatedantibody,aphospho-specificAnti-phospho-HistoneH2A.X(Ser139)-AlexaFluor®488conjugatedantibodytomeasurelevelsofHistoneH2A.X.ThiskitisdesignedtodetecttheextentofHistoneH2A.XpathwayactivationbymeasuringH2A.Xphosphorylationinanygivencellpopulation.ThekitprovidesasensitiveandvaluabletooltostudythefactorsthatinduceDNAdamageand/oraffectDNArepair,andallowonetoexplorethelinkagebetweenDNAdamage,cellcyclecheckpoints,andinitiationofapoptosis.

      IonizingrADIation(IR)andmanychemotherapeuticagentslikeetoposidekillcancercellsbyinductionofDNADSBs.Severalreportsshowthatthelevelofgamma-H2A.XasdetectedbyflowcytometrycorrelateswiththenumberofDNAstrandbreaks,tothelevelofcelldeathandradiosensitivity.H2A.XphosphorylatesinresponsetoaDNAdamagingreagent(e.g.Etoposide)orUVlight,anditsactivationclearlyindicatesthatDSBshaveoccurred.UnderstandingwhenDSBstakeplacecanhelpresearchersunderstandthemechanismsinvolvedinDNArepairandtheDNAdamageresponse.

      TheantibodyprovidedinthekithasbeencarefullytitratedtoensuretheABIlitytomeasurephospho-HistoneH2A.Xforaccuratedeterminationofproteinactivation.Sufficientreagentsareprovidedtoperform100tests.Detailedassayinstructionsareincludedtoassistinanalysisandtoensurethecorrectcellconcentrationisobtainedduringacquisitionofsampledata.
      AlternateNames
      • FlowCellect
      BackgroundInformationH2A.XisamemberofthehistoneH2Afamily.HistoneH2A.XresidesdownstreamoftheDNAdamagekinasesignalingcascade.PhosphorylationofHistoneH2A.Xatserine139isanimportantindicatorofDNAdamage.AsthelevelofDNAdamageincreases,thelevelofphosphoHistoneH2A.X(alsoknownasγH2AX)increases,accumulatingatthesitesofDNAdamage.ThisaccumulationofphosphoHistoneH2A.XisoftenusedtoindicatethelevelofDNAdamagepresentwithinthecell.H2AXisalsoresponsIBLeforrecruitingresponseproteinstothesiteofDNAdamageandmayplayaroleinDNArepair.

      Inall,acomprehensiveunderstandingofHistoneH2A.XactivityandunderstandingtheconsequenceofitsactivationcanprovidetheresearcherwithusefulinformationwhichwillbeimportantinunderstandingtheintrinsicnatureoftheDNAdamageresponse.Millipore’sFlowCellectHistoneH2A.XPhosphorylationAssayKitisdesignedtoallowtheresearchertomonitorandaccuratelymeasurephospho-specificHistoneH2A.Xactivationinapopulationofcells.

      AllFlowCellectkitsareoptimizedonguava®benchtopflowcytometers.FlowCellectkitscanbeusedonanyflowcytometerfollowingthesameprotocolprovidingresearchersareliableandfullyvalidatedsolutiontostudytheHistoneH2A.Xsignalingpathwayrightinthecomfortoftheirownlab.Thedirectlyconjugatedantibodyprovidedinthekithasbeencarefullytitratedandoptimizedtoensuremaximalperformancealleviatingtheneedforanyadditionaloptimization.Thiskitcontainsoptimizedfixation,permeabilization,wash,andassaybufferstoprovideresearcherswithacompletesolutionforHistoneH2A.Xsignalinganalysis.
      ProductInformation
      Components
      • 1.20XAnti-HistoneH2A.X-AlexaFluor®488:(PartNo.CS208216)Onevialcontaining600µL.
      • 2.FixationBuffer:(PartNo.CS202122)Onebottlecontaining13mL.
      • 3.10XWashBuffer:(PartNo.CS202123)Onebottlecontaining13mL.
      • 4.5XAssayBuffer:(PartNo.CS202124)One55mLbottle
      • 5.1XPermeabilizationBuffer:(PartNo.CS203284)Onebottlecontaining14mL.
      DetectionmethodFluorescent
      StorageandShippingInformation
      StorageConditions4-8°Cforantibodiesandbuffers
      Applications
      ApplicationThisFlowCellectHistoneH2A.XPhosphorylationAssayKitforFlowCytometryincludesonedirectlyconjugatedantibody,aphospho-specificAnti-phospho-HistoneH2A.X(Ser139)-AlexaFluor488conjugatedantibodytomeasurelevelsofHistoneH2A.X.
      KeyApplications
      • FlowCytometry
      BIOLOGicalInformation
      HostMouse
      SpeciesReactivity
      • Human
      AntibodyTypeMonoclonalAntibody
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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