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Millipore/FCCS025210 | FlowCellect™ PI3K-mTOR Signaling Cascade kit /FCCS025210/25 Tests / Kit

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货号: FCCS025210

品牌: Millipore

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Description
CatalogueNumber	FCCS025210
TradeName	FlowCellect
Description	FlowCellect™PI3K-mTORSignalingCascadekit
Overview	SignalingthroughthePI3K/Akt/mTORpathwayisconstitutivelyactiveinmanycancercellsduetothelossofPTEN(tumorsuppressor),exposuretocarcinogens,activationofgrowthreceptors,aswellasmutationsoramplificationsofPI3KorAkt.Therelevanceofthispathwayindifficulttotreatcancersmakesthesessignalingnodesprimetargetsinadvancinganticancertherapies. Millipore’sFlowCellect™PI3K-mTORAnalysisKitincludestwodirectlyconjugatedphospho-specificsignalingantibodieswhichareoptimizedformulti-colorflowcytometryapplicationstoanalyzethemTORpathwayingreatdetail.ThephosphorylationofAktisindicativeoftheupstreamPI3Ksignaling,markingthecellsinitiationintoproliferationorcellsurvival.Phosporylated-RibosomalS6proteinisindicativeofdownstreammTORandp70S6KsignalingleADIngtoproteintranslation. Byprovidingbothoftheseantibodiesinonekit,theresearcherhasthecompletesolutionwhenexaminingthemTORpathwaywhichisbeneficialfortheelucidationofdrugeffectivenessintheircancerresearch.
AlternateNames	FlowCellect
BackgroundInformation	Examinationofcellsignalingpathwaysandmonitoringtheiractivationstatushavebeenextremelyimportantforresearcherstounderstandthedetailedmechanismsofcellularfunctionsandthecauseofvariousdiseases.Manysignaltransductionpathwayshavebeenimplicatedtoleadtomultipleoutcomessuchasapoptosis,celldifferentiation,cellgrowthandcellproliferation,allofwhichhavebeenextensivelystudiedforthetreatmentofvariouscancersandautoimmunediseases. Thestudyofcellsignalingpathwaysarenowmadeeasierwiththeuseofactivationstatus-specificandphospho-specificantibodies.Measurementofproteinphosphorylationwithphospho-specificantibodieshasgiveninsightintokinasesignalingcascades[Krutzik,P.O.etal.(2003].Multi-parameterphosphoflowcytometryisapowerfultoolforstudyingmultiplepathwaysinamixedcellpopulationatthesametime. Milliporeoffersarangeofnovelantibodybasedmulti-parameterFlowCellect™kitswhicharespecificallydesignedtoaddressthecurrentneedsinphosphoflowcytometryanalysisofcellsignalingpathwaysandcancerresearchinamulti-parametricfashion.Alloftheantibodiesprovidedinthekithavebeendirectlyconjugatedandoptimizedformulti-colorflowcytometryapplications.Byusingacombinationofantibodiesprovidedinthekit,researcherscannowaccuratelymeasurekeysignalingnodesandendpointsactivations.Moreover,researcherscanalsodissectanyparticularlinearsignalingpathwaycascadebymeasuringtheactivationofseveralcomponentsinatimecoursedependentmanner.ByusingtheFlowCellect™PI3K-mTORSignalingCascadekit,theresearchercannowstudymTORingreaterdetailanditsrelationshipingeneralcellfunctionsuchascellproliferation,apoptosisandcancer. AllFlowCellect™kitsareoptimizedonthebench-topGuava®flowcytometrysystems,whichsavesvaluabletimeandsamplevolume.Allkitscontainoptimizedfixation,permeABIlization,washandflowbufferstoprovideresearcherswithacompletesolutionforsimultaneousdetectionofmultiplepathwayactivations.WiththeGuavaplatformandFlowCellect™kits,onecanfinallyhaveaneasy,reliableandfullyvalidatedsolutiontostudythecomplexcellsignalingpathwaysrightinthecomfortofyourownlab.

ProductInformation
Components	1.20XAnti-phospho-RibosomalProteinS6(Ser235)PerCPconjugateMonoclonalAntibody:(PartNo.CS203308)One150µLvial 2.20XAnti-phospho-Akt1/PKBα(Ser473)AlexaFluor488conjugateMonoclonalAntibody:(PartNo.CS203310)One150µLvial 3.FixationBuffer:(PartNo.CS202122)One13mLbottle 4.10XWashBuffer:(PartNo.CS202123)One13mLbottle 5.5XAssayBuffer:(PartNo.CS202124)One55mLbottle 6.1XPermeabilizationBuffer:(PartNo.CS203284)Two13mLbottles
Detectionmethod	¹²⁵IFluorescent

ProductInformation

Components

1.20XAnti-phospho-RibosomalProteinS6(Ser235)PerCPconjugateMonoclonalAntibody:(PartNo.CS203308)One150µLvial
2.20XAnti-phospho-Akt1/PKBα(Ser473)AlexaFluor488conjugateMonoclonalAntibody:(PartNo.CS203310)One150µLvial
3.FixationBuffer:(PartNo.CS202122)One13mLbottle
4.10XWashBuffer:(PartNo.CS202123)One13mLbottle
5.5XAssayBuffer:(PartNo.CS202124)One55mLbottle
6.1XPermeabilizationBuffer:(PartNo.CS203284)Two13mLbottles

Detectionmethod

¹²⁵IFluorescent

StorageandShippingInformation
StorageConditions	4-8°Cforantibodiesandbuffers

Applications
Application	ThisFlowCellectPI3K-mTORAnalysisKitincludestwodirectlyconjugatedphospho-specificsignalingantibodieswhichareoptimizedformulti-colorflowcytometryapplicationstoanalyzethemTORpathwayingreatdetail.
KeyApplications	FlowCytometry

BIOLOGicalInformation
Host	Rabbit/Murine
SpeciesReactivity	Human
AntibodyType	MonoclonalAntibody

PhysicochemicalInformation

Dimensions

MaterialsInformation

MaterialsInformation

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