Concentration | 1mg/mL |
Purity | Approximately95%,asdeterminedbySDS-PAGE.Adoublebandof220kDaispresentunderreducedconditions.hFNispurifiedbyaffinitychromatographyongelatinagarose,followedbychromatographyonheparin-agarose.Plasmafromdonorshasbeenscreened,andshowntobenegativeforHIV,HTLV,HepatitisBandC. |
Source | Humandonorplasma. |
EntrezGeneNumber | |
EntrezGeneSummary | Thisgeneencodesfibronectin,aglycoproteinpresentinasolubledimericforminplasma,andinadimericormultimericformatthecellsurfaceandinextracellularmatrix.Fibronectinisinvolvedincelladhesionandmigrationprocessesincludingembryogenesis,woundhealing,bloodcoagulation,hostdefense,andmetastasis.Thegenehasthreeregionssubjecttoalternativesplicing,withthepotentialtoproduce20differenttranscriptvariants.However,thefull-lengthnatureofsomevariantshasnotbeendetermined. |
GeneSymbol | - FN1
- DKFZp686O13149
- DKFZp686H0342
- DKFZp686I1370
- MSF
- FN
- CIG
- DKFZp686F10164
- LETS
- FINC
|
UniProtNumber | |
UniProtSummary | FUNCTION:SwissProt:P02751#Fibronectinsbindcellsurfacesandvariouscompoundsincludingcollagen,fibrin,heparin,DNA,andactin.Fibronectinsareinvolvedincelladhesion,cellmotility,opsonization,woundhealing,andmaintenanceofcellshape.InteractionwithTNRmediatesinhibitionofcelladhesionandneuriteoutgrowth(Bysimilarity). SIZE:2386aminoacids;262607Da SUBUNIT:Mostlyheterodimersormultimersofalternativelysplicedvariants,connectedby2disulfidebondsnearthecarboxylends;toalesserextenthomodimers.InteractswithFBLN1,AMBP,TNR,LGALS3BPandCOL13A1. SUBCELLULARLOCATION:Secreted,extracellularspace,extracellularmatrix. TISSUESPECIFICITY:PlasmaFN(solubledimericform)issecretedbyhepatocytes.CellularFN(dimericorcross-linkedmultimericforms),madebyfibroblasts,epithelialandothercelltypes,isdepositedasfibrilsintheextracellularmatrix.Ugl-Y1,Ugl-Y2andUgl-Y3arefoundinurine.DEVELOPMENTALSTAGE:Ugl-Y1,Ugl-Y2andUgl-Y3arepresentintheurinefrom0to17yearsofage. PTM:Sulfated. SIMILARITY:SwissProt:P02751##Contains12fibronectintype-Idomains.&Contains2fibronectintype-IIdomains.&Contains16fibronectintype-IIIdomains. |
StemCellType | - HumanEmbryonicStemCells
- MesenchymalStemCells
- NeuralStemCells
- HematopoieticStemCells
- EpithelialCells
- PancreaticStemCells
- InducedPluripotentStemCells
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