Azidomethyl-dU(AmdU)isanucleosidethatcontainsanazidegroup.Thestructureofthenucleosideissimilartothymidine,anditisincorporatedintonascentDNAbycellularpolymerases,similarlytoEdU.
UnlikeEdUthatneedstobefurthermodifiedwithazidesinthepresenceofcoppercatalyst,AmdUcanalsoreactintheabsenceofcoppercatalystusingspAACClickchemistrywithstrainedcycloalkynes,suchascyclooctynes.ThisenablesdetectionofnascentDNAinbenign,copperfreeconditions.
Generalproperties
Appearance: | white/offwhitesolid |
Molecularweight: | 283.24 |
Molecularformula: | C10H13N5O5 |
IUPACname: | 5-Azidomethyl-2"-deoxyuridine |
Solubility: | goodinwater,alcohols,DMSO,DMF |
Qualitycontrol: | NMR1H,HPLC-MS(95%) |
Storageconditions: | Storage:24monthsafterreceivalat-20°Cinthedark.Transportation:atroomtemperatureforupto3weeks.Avoidprolongedexposuretolight.Desiccate. |