StorageConditions | Storeat2°to8°C,forupto6months.
ChemiconHRPconjugatedantibodiesmaybedilutedupto1:16,000inEPAIIBuffer.Dilutedconjugatesarestableforatleast3monthswhenstoredat2°to8°C.ThebufferissuitablefordirectuseinELISA.
EPAIIBufferiscompatIBLewithblockingagentssuchasskimmilkpowder,Tween20andnormalserum.HoweverHRPconjugatesshouldnotbestoredinthepresenceoftheseblockingagents.Theyshouldbeaddedjustpriortouseintheassay.
PRECAUTIONS:
HRPconjugatesshouldbedilutedandstoredundercleanconditionstoreducethelevelofmicrobialchallenge.
SodiumazidemustNOTbeaddedtoEPAIIBuffer.
AvoidcontaminationofHRPconjugatesolutionswithheavymetalions,aromaticchlorocarbonsorhypochlorousacids.Avoidcontainersmadeofsodaglassorpolystyrene.Storageofdilutedconjugatesinborosilicateglassisrecommended.
EfficientwashingstepsshouldfollowHRPconjugateincubations.InELISA,atleastfourthoroughwashcyclesarerecommended. |