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Millipore/ECM580 | QCM Haptotaxis Cell Migration Assay -Fibronectin, 24-well, colorimetric/ECM580/1 kitSufficient for an
  • Millipore/ECM580 | QCM Haptotaxis Cell Migration Assay -Fibronectin, 24-well, colorimetric/ECM580/1 kitSufficient for an

Millipore/ECM580 | QCM Haptotaxis Cell Migration Assay -Fibronectin, 24-well, colorimetric/ECM580/1 kitSufficient for an

价格: ¥5724.00 市场价: 9540.00

货号: ECM580
品牌: Millipore
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    • Description
      CatalogueNumberECM580
      ReplacesECM500
      BrandFamilyChemicon®
      TradeName
      • QCM
      • Chemicon
      DescriptionQCMHaptotaxisCellMigrationAssay-Fibronectin,24-well,colorimetric
      OverviewAlsoavailable:CellComb™ScratchAssay!Getbiochemicaldatafromascratchassay!ClickHere

      Introduction
      TheCHEMICONQCM™HaptotaxisCellMigrationAssay-Fibronectin,Colorimetricisidealforthestudyofepithelialandfibroblastcellhaptotaxis.Thequantitativenatureofthisassayisespeciallyusefulforlargescalescreeningofpharmacologicagents.BSA-coatedcontrolchambersprovideanappropriatemigrationcontrol.The8μmporesizeofthisassay"sBoydenchambersisnotappropriateforthestudyoflymphocytemigration.
      •Eachkitprovidessufficientmaterialsfortheevaluationof12samples.
      •Pleaserefertotheproductinsertforassaydetails.

      TheCHEMICONQCM™HaptotaxisCellMigrationAssay-Fibronectin,Colorimetricassayisintendedforresearchuseonly;notfordiagnosticapplications.
      MaterialsRequiredbutNotDelivered1.PrecisionPipettes:sufficientforaliquotingcells.
      2.Harvestingbuffer:EDTAortrypsinbasedcelldetachmentbuffer,orothercelldetachmentformulationsasoptimizedbyindividualinvestigators.Chemicon"sready-to-usenon-mammaliandetachmentsolution,Accutase™(Cat.No.SCR005)canalsobeused.
      3.Tissueculturegrowthmediumappropriateforsubjectcells,suchasDMEMcontaining5%FBS.
      4.QuenchingMedium:serum-freemedium,suchasDMEM,MEMetccontaining5%BSA.Mustcontaindivalentcations(Mg2+,Ca2+)sufficientforquenchingEDTAinharvestingbuffer.
      5.SterilePBSorHBSStowashcells.
      6.Distilledwater
      7.(Optional)Chemoattractantorpharmacologicalagentaddedtoculturemedium.
      8.Lowspeedcentrifugeandtubesforcellharvesting.
      9.CO2incubatorappropriateforsubjectcells.
      10.Hemocytometerorothermeansofcountingcells.
      11.TrypanblueorequivalentviABIlitystain.
      12.Microplatereader(540-570nmdetection)orspectrophotometer.
      13.Sterilecellculturehood
      14.(Optional)Graduatedocular(calibrated),orautomatedmethodforcountingstainedcellsonamembrane.
      15.Shaker
      ProductInformation
      Components
      • FibronectinTestPlate:(PartNo.2005689)One24-wellcultureplate,containing12humanFN-coatedBoydenchambers,sufficientfortheevaluationof12testsamples.
      • BSAControlPlate:(PartNo.2005791)One24-wellcultureplate,containing12BSA-coatedBoydenchambers,sufficientfortheevaluationof12controls.
      • CellStainSolution*:(PartNo.20294)Onevial-10mL.
      • ExtractionBuffer:(PartNo.20295)Onevial-10mL.
      • 24wellStainExtractionPlate:(PartNo.2005871).Oneplate.
      • 96wellStainQuantitationPlate:(PartNo.2005870).Oneplate.
      • Swabs:(PartNo.10202)50each.
      • Forceps:(PartNo.10203)1pair
      • *CellStainSolutioncontainsasmallamountofcrystalviolet,whichistoxicifswallowedorinhaled,andmaycauseirritationtotheeyes,respiratorysystem,andskin.Handlewithcaution.
      DetectionmethodChromogenic
      StorageandShippingInformation
      StorageConditionsStoreat4°Cforuptooneyearafterdateofpurchase.
      Applications
      ApplicationTheQCMHaptotaxisCellMigrationAssay,24-wellplate-Fibronectin,Colorimetricisidealforthestudyofepithelial&fibroblastcellhaptotaxis.
      BIOLOGicalInformation
      SpeciesReactivity
      • Vertebrates
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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