当前位置 ： Millipore >>> Millipore/ECM340 | Human sVCAM-1 ELISA/ECM340/1 kit
Millipore/ECM340 | Human sVCAM-1 ELISA/ECM340/1 kit
  • Millipore/ECM340 | Human sVCAM-1 ELISA/ECM340/1 kit

Millipore/ECM340 | Human sVCAM-1 ELISA/ECM340/1 kit

价格: ¥7560.00 市场价: 12600.00
打印产品信息
货号: ECM340
品牌: Millipore
规格
数量
库存(0)
特别 提示
代购产品：无质量问题不接受退换货，下单前请仔细核对信息。
下单后请及时联系客服核对商品价格，订单生效后再付款。
立即购买 加入购物车

收藏商品
资深产品顾问
咨询顾问

全国免费服务热线

4000-520-616

  • 自营商城 一站式服务
  • 厂家直采 剔除溢价
  • 品质甄选 正品保证
  • 严控流程 只做188精品
  • 极速物流 如约送货
  • 详情
  • 使用说明
  • 常见问题
    • Description
      CatalogueNumberECM340
      BrandFamilyChemicon®
      TradeName
      • Chemicon
      DescriptionHumansVCAM-1ELISA
      OverviewVascularcelladhesionmolecule-1(VCAM-1,CD106)isamemberoftheimmunoglobulingenesuperfamily.TwoformsofVCAM-1withsixorsevenextracellularIg-likedomainsariseduetoalternativesplicingfromaseven-domainVCAM-1(7DVCAM-1).7DVCAM-1isthedominantformexpressedbyculturedhumanendothelialcells[Cybulskyetal.,1991;Hessionetal.,1991].Homologyofdomains1through3withdomains4through6suggestsageneduplicationevent[Cybulskyetal.,1991;Hessionetal.,1991].TheCDNAof7DVCAM-1predictsacoreproteinofapproximately81kDawithsevensitesforN-linkedglycosylation.Uponcompleteglycosylationthematureproteinhasamolecularweightofapproximately102kDa.Thisobservationisingeneralagreementwiththe110kDaproteinimmunoprecipitatedfromcytokine-activatedendothelium[Carlosetal.,1990;Riceetal.,1990].

      VCAM-1appearstohavebeenhighlyconservedthroughevolution.BothratandmouseVCAM-1arehighlyhomologousattheproteinleveltothehumanVCAM-1(77%and76%,respectively)[Hessionetal.,1992].

      VCAM-1supportstheadhesionoflymphocytes,monocytes,naturalkillercells,eosinophilsandbasophils,throughitsinteractionwithintegrina4b1(VLA-4).VCAM-1/VLA-4interactionmediatesfirmadherenceofcirculatingnon-neutrophilicleukocytestoendothelium[Shararetal.,1995].VCAM-1alsoparticipatesinleukocyteadhesionoutsideofthevasculature,mediatingprecursorlymphocyteadhesiontobonemarrowstromalcells[Ryanetal.1991]andBcellbindingtolymphnodefolliculardendriticcells[Feedmanetal.,1992].VCAM-1isnotconstitutivelyexpressedonendothelium,butcanbeup-regulatedinvitroinresponsetoLPS,TNF-a,andIL-1[Carlos&Harlan,1990;Osbornetal.,1989],aswellastointerferon-gandIL-4[Masinovskyetal.1990].VCAM-1isalsopresentontissuemacrophages,dendritecells,bonemarrowfibroblasts,myoblastsandmyotubes.

      AsolubleformofVCAM-1hasbeendescribed[DeMaeyer&DeMaeyer-Buignard,1995;Lobbetal.,1991],andsolubleVCAM-1hasbeenfoundinserum[Gearingetal.,1995].IncreasedlevelsofsolubleVCAM-1canbedetectedinseveraldiseasestates,includingcancer[Banksetal.,1993;Fortisetal.,1995;Gearing&Newman,1993;Laietal.,1995],autoimmunedisorders[Gearing&Newman,1993;Doreduffyetal.,1995;Gruschwitzetal.,1995;Masonetal.,1993;Mrowka&Sieberth,1995;Spronketal.,1994;Wellicomeetal.,1993],infections[Jakobsenetal.,1994]andinflammations[Gearing&Newman,1993;Mrowka&Sieberth,1995;Adamsetal.,1995;Limetal.,1995].

      TestPrinciple:

      Ananti-VCAM-1monoclonalantibodyisadsorbedontomicrowells.

      SolubleVCAM-1presentinasampleorstandardthenbindstoantibodiesadsorbedtothemicrowells.Amixtureofbiotin-conjugatedmonoclonalanti-VCAM-1antibodyandStreptavidin-HRPconjugateisadded.Biotinylatedanti-VCAM-1antibodybindstoVCAM-1capturedbythefirstantibody.

      Streptavidin-HRPbindstothebiotinylatedanti-VCAM-1.Unboundbiotinylatedanti-VCAM-1andStreptavidin-HRPconjugateisremovedwithawashstepandHRPsubstratesolutionisaddedtothewells.

      ThepairofmonoclonalantibodiesusedinthisELISAdetectthesolubleformofVCAM-1presentinserum,plasma,urine,andotherBIOLOGicalfluids.

      Anamountofcoloredproductisformed,proportionaltotheamountofsolubleVCAM-1presentinthesample.Thereactionisterminatedbyadditionofacidandabsorbanceismeasuredat450nm.AstandardcurveispreparedfromsixsolubleVCAM-1standarddilutionsandtheVCAM-1sampleconcentrationisdetermined.

      Application:

      TheVCAM-1ELISAisanenzyme-linkedimmunosorbentassayforquantitativedetectionofsolubleVascularCellAdhesionMolecule-1incellculturesupernatants,serum,plasma,orotherbiologicalfluids.TheVCAM-1ELISAisforresearchuseonly.Notforuseindiagnosticprocedures.
      AlternateNames
      • CD106
      MaterialsRequiredbutNotDelivered-5mLand10mLgraduatedPipettes

      -5μLto1000μLadjustablesinglechannelmicropipetteswith

      disposabletips

      -50μLto300μLadjustablemultichannelmicropipettewithdisposabletips

      -Multichannelmicropipettereservoir

      -Beakers,flasks,cylindersnecessaryforpreparationofreagents

      -Devicefordeliveryofwashsolution(multichannelwashbottleor

      automaticwashsystem)

      -MicrowellstripreadercapableofreADIngat450nm(620nmas

      optionalreferencewavelength)

      -Glass-distilledordeionizedwater

      -Statisticalcalculatorwithprogramtoperformlinearregressionanalysis.
      ProductInformation
      Components
      • ·2aluminumpoucheswith3MicrowellStrips(16wellsperstrip)coatedwithMonoclonalAntibody(murine)tohumanVCAM-1
      • ·1vial(0.1mL)ConjugateMixturecontainingBiotin-ConjugatedAnti-VCAM-1Monoclonal(murine)AntibodymixedwithStreptavidin-HRP*
      • ·2vials(200ng/mLasreconstituted)solubleVCAM-1Standard,lyophilized*
      • ·1viallyophilizedcontrolserum
      • ·1bottle(50mL)WashBufferConcentrate20X(PBSwith1%Tween20)*
      • ·1vial(5mL)AssayBufferConcentrate20X(PBSwith1%Tween20and10%BSA)*
      • ·1vial(7mL)SubstrateSolutionI(tetramethyl-benzidine)
      • ·1vial(7mL)SubstrateSolutionII(0.02%bufferedhydrogenperoxide)
      • ·1vial(12mL)StopSolution(1MPhosphoricAcid,H3PO4),readytouse
      • ·1MicrowellStripHolder
      • ·2AdhesivePlateCovers
      • ·reagentscontaining0.01%thimerosalaspreservative
      DetectionmethodChromogenic
      StorageandShippingInformation
      StorageConditionsPrecautions:

      ·Reagentsareintendedforresearchuseonlyandarenotforuseindiagnosticortherapeuticprocedures.

      ·Donotmixorsubstitutereagentswiththosefromotherlotsorothersources.

      ·Donotusekitreagentsbeyondexpirationdateonlabel.

      ·Donotexposekitreagentstostronglightduringstorageorincubation.

      ·Rubberordisposablelatexglovesshouldbewornwhilehandlingkitreagentsorspecimens.

      ·Somereagentscontainthimerosalaspreservative,whichishighlytoxicbyinhalation,ingestion,orcontactwithskin.ThimerosalisapossIBLemutagenandshouldbehandledaccordingly.

      ·Avoidcontactofsubstratesolutionswithoxidizingagentsandmetal.

      ·Inordertoavoidmicrobialcontaminationorcross-contaminationofreagentsorspecimenswhichmayinvalidatethetestusedisposablepipettetipsand/orpipettes.

      ·Useclean,dedicatedreagenttraysfordispensingtheconjugateandsubstratereagents.

      ·Exposuretoacidswillinactivatetheconjugate.

      ·Glass-distilledwaterordeionizedwatermustbeusedforreagent

      preparation.

      ·Substratesolutionsmustbeatroomtemperaturepriortouse.

      ·Sinceexactconditionsmayvaryfromassaytoassay,astandard

      curvemustbeestablishedforeveryrun.

      ·Bacterialorfungalcontaminationofeitherscreensamplesorreagentsorcross-contaminationbetweenreagentsmaycauseerroneousresults.

      ·Disposablepipettetips,flasksorglasswarearepreferred.Reusableglasswaremustbewashedandthoroughlyrinsedofalldetergentsbeforeuse.

      ·Improperorinsufficientwashingatanystageoftheprocedurewillresultineitherfalsepositiveorfalsenegativeresults.CompletelyemptywellsbeforedispensingfreshWashBuffer,fillwithWashBufferasindicatedforeachwashcycleanddonotallowwellstosituncoveredordryforextendedperiods.

      ·Anyhumananti-mouseIgGantibody(HAMA)presentmayinterferewithassaysutilizingmurinemonoclonalantibodiesleadingtobothfalsepositiveandfalsenegativeresults.HAMAinterferencecanbereducedbyaddingmurineimmunoglobulins(serum,asciticfluid,ormonoclonalantibodiesofirrelevantspecificity)totheSampleDiluent.
      Applications
      ApplicationThisHumansVCAM-1ELISAisusedtomeasure&quantifysVCAM-1levelsinInflammation&Immunology&Neuroscienceresearch.
      KeyApplications
      • ELISA
      BiologicalInformation
      SpeciesReactivity
      • Human
      AnalytesAvailable
      • sVCAM-1
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneisamemberoftheIgsuperfamilyandencodesacellsurfacesialoglycoproteinexpressedbycytokine-activatedendothelium.ThistypeImembraneproteinmediatesleukocyte-endothelialcelladhesionandsignaltransduction,andmayplayaroleinthedevelopmentofartherosclerosisandrheumatoidarthritis.Twoalternativelysplicedtranscriptsencodingdifferentisoformshavebeendescribedforthisgene.
      GeneSymbol
      • VCAM1
      • INCAM-100
      • MGC99561
      • CD106
      • DKFZp779G2333
      • L1CAM
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P19320#Importantincell-cellrecognition.Appearstofunctioninleukocyte-endothelialcelladhesion.Interactswiththebeta-1integrinVLA4onleukocytes,andmediatesbothadhesionandsignaltransduction.TheVCAM1/VLA4interactionmayplayapathophysiologicrolebothinimmuneresponsesandinleukocyteemigrationtositesofinflammation.
      SIZE:739aminoacids;81276Da
      SUBCELLULARLOCATION:Membrane;Single-passtypeImembraneprotein.
      TISSUESPECIFICITY:Expressedoninflamedvascularendothelium,aswellasonmacrophage-likeanddendriticcelltypesinbothnormalandinflamedtissue.
      DOMAIN:SwissProt:P19320EitherthefirstorthefourthIg-likeC2-typedomainisrequiredforVLA4-dependentcelladhesion.
      PTM:Sialoglycoprotein.
      DISEASE:SwissProt:P19320#Mayplayanimportantroleinthegenesisofatherosclerosisandrheumatoidarthritis.
      SIMILARITY:Contains7Ig-likeC2-type(immunoglobulin-like)domains.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
    售后保障
    蚂蚁淘生物188，秉承蚂蚁淘一贯的严谨态度，由蚂蚁淘公司专业人员负责品控、采购、物流、销售、售后，保障正品优质。以“快速好省，为科研提供好产品、好价格”为理念，直接链接原厂家，从全国各地原制造商严格挑选188款科研精品，剔除品牌溢价，188生物新电商，把好的产品带给科研！  力求给你最优质的商品。
  • Q：生物188产品正品保障吗？
    A：生物188质量把控人员具有十年的从业经验，在业界享有良好的口碑；自营商城，直接从厂家采购， 自己的团队负责国际物流和清关，中间没有第三方，所有流程严格把控，100%保证正品，假一罚十。

    Q：下单后可以修改订单吗？
    A：下单后的商品付款之前可以修改；订单付款成功，需要联系我们客服进行修改；客服电话：4000-520-616

    Q：可以开发票吗？
    A：本网站所售商品都是正规清关，均开具16%正规发票，发票金额含配送费金额，另有说明的除外。

    Q：商品几天可以发货？
    A：生物188商品，全部现货销售，付款后即可发货,一般一周内送达！

    Q：如何联系商家？
    A：有任何问题够可以电话咨询我们，全国24小时免费服务热线：4000-520-616 或联系我们的在线客服QQ：1570468124

    Q：收到的商品少了/发错了怎么办？
    A：同个订单购买多个商品可能会分为一个以上包裹发出，可能不会同时送达，建议查看订单详情是否是 部分发货状态；如未收到，可联系在线客服或者致电4000-520-616。

    Q：退换货/维修需要多长时间？
    A：一般情况下，退货处理周期为客户收到产品一个月内（以快递公司显示签收时间为准），包装规格、 数量、品种不符，外观毁损、短缺或缺陷，请在收到货24小时内申请退换货；特殊商品以合同条款为准。

客户服务

在线客服
用户反馈

何为188

极简而严谨，我们仅销售188款生物医学科研用品，款款都是爆款；因为少所以聚焦，聚焦甄选每一款产品，聚焦服务每一位客户!

关注我们 :

点击QQ联系我们
生物188微信

关注188微信公众号

获取最新优惠活动通知

  • 品质甄选，正品保证

  • 自营电商，厂家直采

  • 极简主义，188精品

常见问题 | 购买相关 | 配送与验收 | 支付问题 | 售后与服务 | 服务协议 | 隐私政策 | 何为188 | 中国智造 | 联系我们
友情链接： arp1产品 | Polysciences第二代聚合物转染 | Lifesci价格 | MedKoo206488 | AvaChem华东代理 | eenzyme品牌代理 | Moltox代理 | 蚂蚁淘 | 快速-毒品

公司版权所有 © 2005-2021 苏州蚂蚁淘生物科技有限公司 苏ICP备17049038号-10