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Millipore/ECM300 | QuantiMatrix Human Fibronectin ELISA/ECM300/1 kit
  • Millipore/ECM300 | QuantiMatrix Human Fibronectin ELISA/ECM300/1 kit

Millipore/ECM300 | QuantiMatrix Human Fibronectin ELISA/ECM300/1 kit

价格: ¥7032.00 市场价: 11720.00
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货号: ECM300
品牌: Millipore
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    • Description
      CatalogueNumberECM300
      BrandFamilyChemicon®
      TradeName
      • Quantimatrix
      • Chemicon
      DescriptionQuantiMatrixHumanFibronectinELISA
      OverviewTheQuantiMatrix™ELISAkitforthedeterminationofsolublehumanfibronectinprovidessensitivitydownto10-20ng/mLasdeterminedfromculturesupernatantsandpurifiedproteinsamples.

      TheassayisintheformatofacompetitiveinhibitionELISA.Humanfibronectinispre-coatedonthewellswiththeexceptionofrowH,whichservesasareference.Standardsandsamplesaredilutedandpre-incubatedwith

      polyclonalrabbitantibodytohumanfibronectin.Thepolyclonalantibodybindstofibronectininthestandarddilutions,andinthesample,ifpresent.Themixtureisthentransferredtothehumanfibronectin-coatedplate.Freerabbitanti-humanfibronectinbindstothefibronectinontheplate.Goatanti-rabbitIgGHRPconjugatereactswithboundrabbitanti-fibronectin.WhenHRPsubstrateisadded,abluecolordevelops.Thereactionisstoppedbytheadditionofanacid,changingthecolortoyellow.ThiscolorisquantitatedusinganELISAreader;theintensityofthecolorisinverselyproportionaltotheamountoffibronectinintheoriginalsample.Astandardcurveisconstructedandsamplevaluesareinterpolated.
      BackgroundInformationThedeterminationoflevelsofextracellularmatrixmoleculesisbecomingmoreimportanttounderstandingalterationsinmatrixfunctionduringdevelopmentandtumOrigenesis.Questionsofmatrixgeneregulationandfunctionrequireasensitivemeansofdeterminingthelevelsofproteinexpressionbycells.TheQuantiMatrix™ELISAkitforthedeterminationofsolublehumanfibronectinprovidessensitivitydownto10-20ng/mLasdeterminedfromculturesupernatantsandpurifiedproteinsamples.

      TheassayisintheformatofacompetitiveinhibitionELISA.Humanfibronectinispre-coatedonthewellswiththeexceptionofrowH,whichservesasareference.Standardsandsamplesaredilutedandpre-incubatedwithpolyclonalrabbitantibodytohumanfibronectin.Thepolyclonalantibodybindstofibronectininthestandarddilutions,andinthesample,ifpresent.Themixtureisthentransferredtothehumanfibronectin-coatedplate.Freerabbitanti-humanfibronectinbindstothefibronectinontheplate.Goatanti-rabbitIgGHRPconjugatereactswithboundrabbitanti-fibronectin.WhenHRPsubstrateisadded,abluecolordevelops.Thereactionisstoppedbytheadditionofanacid,changingthecolortoyellow.ThiscolorisquantitatedusinganELISAreader;theintensityofthecolorisinverselyproportionaltotheamountoffibronectinintheoriginalsample.Astandardcurveisconstructedandsamplevaluesareinterpolated.
      ProductInformation
      Components
      • QuantiMatrixTMFibronectinCoatedStrips(PartNo.60103)-1x96-wellstripplate
      • HumanFibronectinStandard(PartNo.60109)-1lyophilizedbottle;reconstituteaccordingtotheprotocolonpage2
      • SampleDiluent(PartNo.60095)-1x15mLbottleof1%BSAinPBS+0.01%Thimerosal
      • Goatanti-RabbitIgG-HRPConjugate(QM2)(PartNo.60110)-1bottle;readytouse
      • Rabbitanti-HumanFibronectin(PartNo.60111)-1bottlepre-dilutedpolyclonalantibody;readytouse
      • TMB/ESubstrate(PartNo.60096)-1bottle;readytouse
      • StopSolution(PartNo.60193)-1bottle;(0.5MHClsolution)
      • WashBufferConcentrate(PartNo.60245)-1x100mLbottle;providesfinalconcentrationsof0.05%Tween-20and0.01%Thimerosalwhenaddedto
      • 900mLofdeionizedwaterasdescribedinstep1ofprotocolonpage2.
      DetectionmethodChromogenic
      StorageandShippingInformation
      StorageConditionsStorethekitandallcomponentsat2-8°C.

      PRECAUTIONS:

      HumanfibronectinwaspurifiedfromhumanplasmathattestednegativeforHBsAgandHIV.However,allmaterialsshouldbehandledcarefullyandinaccordancewithgoodlaboratorypractices.

      Theinstructionsprovidedhavebeendesignedtooptimizeperformance.Deviationsfromthesuggestedproceduremayresultinsub-optimalperformanceofthekitandfailuretoproduceaccuratedata.

      Donotmixorsubstitutereagentsfromdifferentkitlots.
      Applications
      ApplicationTheQuantiMatrixELISAkitforthedeterminationofsolublehumanfibronectinprovidessensitivitydownto10-20ng/mLasdeterminedfromculturesupernatants&purifiedproteinsamples.
      KeyApplications
      • ELISA
      BIOLOGicalInformation
      SpeciesReactivity
      • Human
      AnalytesAvailable
      • Fibronectin
      EntrezGeneNumber
      EntrezGeneSummaryThisgeneencodesfibronectin,aglycoproteinpresentinasolubledimericforminplasma,andinadimericormultimericformatthecellsurfaceandinextracellularmatrix.Fibronectinisinvolvedincelladhesionandmigrationprocessesincludingembryogenesis,woundhealing,bloodcoagulation,hostdefense,andmetastasis.Thegenehasthreeregionssubjecttoalternativesplicing,withthepotentialtoproduce20differenttranscriptvariants.However,thefull-lengthnatureofsomevariantshasnotbeendetermined.
      GeneSymbol
      • FN1
      • DKFZp686O13149
      • DKFZp686H0342
      • DKFZp686I1370
      • MSF
      • FN
      • CIG
      • DKFZp686F10164
      • LETS
      • FINC
      UniProtNumber
      UniProtSummaryFUNCTION:SwissProt:P02751#Fibronectinsbindcellsurfacesandvariouscompoundsincludingcollagen,fibrin,heparin,DNA,andactin.Fibronectinsareinvolvedincelladhesion,cellmotility,opsonization,woundhealing,andmaintenanceofcellshape.InteractionwithTNRmediatesinhibitionofcelladhesionandneuriteoutgrowth(Bysimilarity).
      SIZE:2386aminoacids;262607Da
      SUBUNIT:Mostlyheterodimersormultimersofalternativelysplicedvariants,connectedby2disulfidebondsnearthecarboxylends;toalesserextenthomodimers.InteractswithFBLN1,AMBP,TNR,LGALS3BPandCOL13A1.
      SUBCELLULARLOCATION:Secreted,extracellularspace,extracellularmatrix.
      TISSUESPECIFICITY:PlasmaFN(solubledimericform)issecretedbyhepatocytes.CellularFN(dimericorcross-linkedmultimericforms),madebyfibroblasts,epithelialandothercelltypes,isdepositedasfibrilsintheextracellularmatrix.Ugl-Y1,Ugl-Y2andUgl-Y3arefoundinurine.DEVELOPMENTALSTAGE:Ugl-Y1,Ugl-Y2andUgl-Y3arepresentintheurinefrom0to17yearsofage.
      PTM:Sulfated.
      SIMILARITY:SwissProt:P02751##Contains12fibronectintype-Idomains.&Contains2fibronectintype-IIdomains.&Contains16fibronectintype-IIIdomains.
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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