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Millipore/ECM201 | QCM 3 µm Endothelial Cell Migration Assay - Fibronectin, Fluorometric/ECM201/1 kitSufficient for 12 a
  • Millipore/ECM201 | QCM 3 µm Endothelial Cell Migration Assay - Fibronectin, Fluorometric/ECM201/1 kitSufficient for 12 a

Millipore/ECM201 | QCM 3 µm Endothelial Cell Migration Assay - Fibronectin, Fluorometric/ECM201/1 kitSufficient for 12 a

价格: ¥5988.00 市场价: 9980.00

货号: ECM201
品牌: Millipore
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    • Description
      CatalogueNumberECM201
      TradeName
      • QCM
      • Chemicon
      DescriptionQCM3µmEndothelialCellMigrationAssay-Fibronectin,Fluorometric
      OverviewAlsoavailable:CellComb™ScratchAssay!Getbiochemicaldatafromascratchassay!ClickHere

      Introduction
      Angiogenesisisafundamentalprocessinvolvingthegrowthofnewbloodvesselsfrompre-existingvessels.Itisimportantindevelopmentandwoundhealing,aswellaspathologicdiseasessuchasdiabeticretinopathyandcancer.Duringangiogenesis,endothelialcellsneedtomoveoutofexistingvessels,migrateintonewareas,proliferateandassembleintonewcapillaries.Themigrationofendothelialcellsisregulatedbymanyangiogenicfactorsandanti-angiogenicfactors.Itiscriticalforresearcherstounderstandthemechanismsofendothelialcellmigration.

      Millipore"s3μmQCM™EndothelialCellMigrationAssay–Fibronectin,FluorometricprovidesaquickandefficientsystemtostudytheABIlityofcompoundstoinduceorinhibitendothelialcellmigration.Thisassayalsoallowsscreeningofpharmacologicalagents,evaluationofintegrinsorotheradhesionreceptorsresponsIBLeforendothelialcellmigration,analysisofgenefunctionintransfectedcells,anddeterminationofECMproteininvolvementincellmovement.

      Thisversatileassaypermitscountingofindividualmigratorycells,and,moreimportantly,allowsquantitativeanalysisbyopticaldensity(OD)usingastandardmicroplatereader.Thisconvenientassayallowslargescalescreeningandquantitativecomparisonofmultiplesamplesandincludesindividualmigrationcontrolsforeachsample.
      MaterialsRequiredbutNotDelivered1.Endothelialcells(forexample:HUVECcells),andcellculturemedium(forexample:EGM-2).
      2.Serum-freemedium,suchasserum-freeEGM-2containing0.5%BSA.
      3.SterilePBSorHBSStowashcells.
      4.Distilledwater
      5.(Optional)Chemoattractantorpharmacologicalagentforadditiontoculturemedium.
      6.Lowspeedcentrifugeandtubesforcellharvesting
      7.CO2incubatorappropriateforsubjectcells
      8.Hemocytometerorothermeansofcountingcells.
      9.Trypanblueorequivalentviabilitystain
      10.Fluorescenceplatereader.


      ProductInformation
      Components
      • 1.FibronectinTestPlate:One24-wellcultureplate,containing12humanFN-coatedBoydenchambers,sufficientfortheevaluationof12testsamples.
      • 2.BSAControlPlate:One24-wellcultureplate,containing12BSA-coatedBoydenchambers,sufficientfortheevaluationof12controls.
      • 3.4XCellLysisBuffer-One16mLbottle
      • 4.CyQuantGRDye®:Onevial75μL
      • 5.24-WellCellCultureTray
      • 6.96-WellStainQuantitationPlate
      • 7.Forceps:1pair
      • 8.Accutase(celldetachmentsolution):One100mLbottle
      DetectionmethodColorimetricFluorescent
      StorageandShippingInformation
      StorageConditions•TheECM201-1kitcomponentsshouldbestoredat2°to8°Cupuntiltheexpirationdateprovidedonthelabels.DONOTFREEZE.
      •Theaccompanying100mlbottleofAccutasealsoincludedinthiskitshouldbestoredat-20°C.
      Applications
      ApplicationThisQCM3μmEndothelialCellMigrationAssay–Fibronectin,Fluorometricisidealforthestudyofendothelialcellmigrationinresponsetoanangiogenicstimulus.
      ApplicationNotesTheMilliporeQCM™3μmEndothelialCellMigrationAssay–Fibronectin,Fluorometricisidealforthestudyofendothelialcellmigrationinresponsetoanangiogenicstimulus.Thequantitativenatureofthisassayisespeciallyusefulforlargescalescreeningofpharmacologicagents.BSA-coatedcontrolchambersprovideanappropriatemigrationcontrol.The3μmporesizeinthisassayisoptimalforendothelialcellssuchasHUVEC,butnotsufficientforfibroblastmigration.TheMilliporeQCM™3μmEndothelialCellMigrationAssay–Fibronectin,Fluorometricassayisintendedforresearchuseonly;notfordiagnosticapplications.
      Eachkitprovidessufficientmaterialsfortheevaluationof12samples.
      BIOLOGicalInformation
      SpeciesReactivity
      • Vertebrates
      PhysicochemicalInformation
      Dimensions
      MaterialsInformation
      MaterialsInformation
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