Description:
ThePh.D.-12PhageDisplayPeptideLibraryisbasedonacombinatoriallibraryofrandomdodecapeptidesfusedtoaminorcoatprotein(pIII)ofM13phage(1–6).Thedisplayedpeptide(12-mer)isexpressedattheN-terminusofpIII,i.e.,thefirstresidueofthematureproteinisthefirstrandomizedposition.Thepeptideisfollowedbyashortspacer(Gly-Gly-Gly-Ser)andthenthewild-typepIIIsequence.Thelibraryconsistsofapproximately109electroporatedsequencesamplifiedoncetoyieldapproximately100copiesofeachsequencein10µlofthesuppliedphage.
CitationsforPhageDisplay
PanningwithapentavalentpeptidelibrarydisplayedonpIII.KitComponents
Thefollowingreagentsaresuppliedwiththisproduct:
| Storeat(°C) | Concentration |
Streptavidin,lyophilized | -20 | |
Biotin | -20 | 10mM |
E.coliK12ER2738 | -70 | |
-28glllSequencingPrimer(22-mer) | -20 | 1pmol/μl |
-96glllSequencingPrimer(20-mer) | -20 | 1pmol/μl |
Ph.D.™-12PhageDisplayPeptideLibrary | -20 | 1x1013pfu/ml |
Streptavidin,lyophilized | -20 | |
Biotin | -20 | 10mM |