Description:
TheNEBNextUltraIILigationModulehasbeenoptimizedtoefficientlyligateDNAadaptorscompatIBLewithIllumina®sequencingtoend-repaired,dA-tailedDNAfragments.ThemoduleisoptimizedforusewiththeNEBNextUltraIIEndRepair/dA-TailingModule(NEB#E7546),andispartoftheUltraIIworkflowwhichenableshighyieldpreparationofhighqualitylibrariesfrom500picogramsto1microgramofinputDNA.
TheNEBNextUltraIILigationModuleisDesignedforusewiththeFollowing:
- NEBNextUltraIIEndRepair/dA-TailingModule(NEB#E7546)
- NEBNextUltraIIQ5®MasterMix(NEB#M0544)
- NEBNextSingleplexorMultiplexOligosforIllumina(NEB#E7350,#E7335,#E7500,#E7600or#E7535)
FunctionalValidation
EachsetofreagentsisfunctionallyvalidatedtogetherthroughconstructionandsequencingofatranscriptomelibraryandsequencedonaDNAlibraryontheIlluminasequencingplatform.
LotControl
Thelotsprovidedaremanagedseparatelyandqualifiedbyadditionalfunctionalvalidation.Individualreagentsundergostandardenzymeactivityandqualitycontrolassays,andalsomeetstringentcriteriaintheadditionalqualitycontrolslistedoneachindividualcomponentpage
ReagentsSupplied
Thefollowingreagentsaresuppliedwiththisproduct:
| Storeat(°C) | Concentration |
NEBNext®Ultra™IILigationMasterMix | -20 | |
NEBNext®LigationEnhancer | -20 | |