Description:
TheNEBNext®Ultra™IIDirectionalRNASecondStrandSynthesisModulehasbeenoptimizedtogeneratedouble-strandedCDNAfromfirst-strandcDNA.ThedsDNAgeneratedbytheNEBNextUltraIIDirectionalRNASecondStrandModulecanbesubsequentlyconvertedtoblunt-endDNAfragmentsusingtheNEBNextUltraEndPrepModule.
TheNEBNextUltraIIDirectionalRNASecondStrandSynthesisModuleisDesignedforUsewiththeFollowing:
NEBNextRNAFirstStrandSynthesisModule(NEB#E7525)
NEBNextUltra™EndRepair/dA-TailingModule(NEB#E7442)
NEBNextUltra™LigationModule(NEB#E7445)
NEBNextSingleplexorMultiplexOligosforIllumina®(NEB#E7350,#E7335or#E7500)
USER™Enzyme(NEB#M5505)Note:ThisisincludedintheNEBNextOligosets;however,ifyouareusingalternativeoligos,itisnecessarytopurchaseseparatelyforthedirectionalmethod.
NEBNextHigh-Fidelity2XPCRMasterMix(NEB#M0541)FunctionalValidation
Eachsetofreagentsisfunctionallyvalidatedtogetherthroughconstructionandsequencingofatranscriptomelibraryandsequencedona.
LotControl
Thelotsprovidedaremanagedseparatelyandqualifiedbyadditionalfunctionalvalidation.Individualreagentsundergostandardenzymeactivityandqualitycontrolassays,andalsomeetstringentcriteriaintheadditionalqualitycontrolslistedoneachindividualcomponentpage
ReagentsSupplied
Thefollowingreagentsaresuppliedwiththisproduct:
| Storeat(°C) | Concentration |
NEBNextSecondStrandSynthesisEnzymeMix | -20 | |
NEBNextSecondStrandSynthesisReactionBufferwithdUTPMix | -20 | 10X |