Description:
TheNEBNextUltraIIEndRepair/dA-TailingModulehasbeenoptimizedtoconvert500pg-1μgoffragmentedDNAtorepairedDNAhaving5´phosphorylated,3´dA-tailedends.ThemoduleisoptimizedforusewiththeNEBNextUltraIILigationModule(NEB#E7595),andispartoftheUltraIIworkflowwhichenableshighyieldpreparationofhighqualitylibrariesfrom500picogramsto1microgramofinputDNA.
ThismoduleiscompatIBLewithIlluminaworkflowsandwithsomeOxfordNanoporeMinION™workflows.FunctionalValidation
EachsetofreagentsisfunctionallyvalidatedtogetherthroughconstructionandsequencingofatranscriptomelibraryandsequencedonaDNAlibraryontheIlluminasequencingplatform.
LotControl
Thelotsprovidedaremanagedseparatelyandqualifiedbyadditionalfunctionalvalidation.Individualreagentsundergostandardenzymeactivityandqualitycontrolassays,andalsomeetstringentcriteriaintheadditionalqualitycontrolslistedoneachindividualcomponentpage
ReagentsSupplied
Thefollowingreagentsaresuppliedwiththisproduct:
| Storeat(°C) | Concentration |
NEBNextUltraIIEndPrepEnzymeMix | -20 | |
NEBNextUltraIIEndPrepReactionBuffer | -20 | |