Description:
TheNEBNextRNAFirstStrandSynthesisModuleisdesignedforsynthesisofCDNAfromRNAfragmentsusingProtoScriptIIReverseTranscriptaseandrandompriming.SinglestrandcDNAcanbeuseddirectlyforsecondstrandcDNAsynthesis.FunctionalValidation
Eachsetofreagentsisfunctionallyvalidatedtogetherthroughconstructionandsequencingofatranscriptomelibraryandsequencedona.
LotControl
Thelotsprovidedaremanagedseparatelyandqualifiedbyadditionalfunctionalvalidation.Individualreagentsundergostandardenzymeactivityandqualitycontrolassays,andalsomeetstringentcriteriaintheadditionalqualitycontrolslistedoneachindividualcomponentpage
ReagentsSupplied
Thefollowingreagentsaresuppliedwiththisproduct:
| Storeat(°C) | Concentration |
NEBNextFirstStrandSynthesisReactionBuffer | -20 | |
RandomPrimers | -20 | |
ProtoScript®IIReverseTranscriptase | -20 | 200,000units/ml |
MurineRNaseInhibitor | -20 | |