The NEBNext Poly(A) mRNA Magnetic Isolation Module is designed to isolate intact poly(A)+ RNA from 1-5µg ofpreviously isolated total RNA. The technology is based on the coupling of Oligo d(T)25 to 1 μm paramagnetic beads which is then used as the solid support for the direct binding of poly(A)+ RNA. Thus, the procedure permits the manual processing of multiple samples and can be adapted for automated high-throughput applications. Additionally, magnetic separation technology permits elution of intact mRNA in small volumes eliminating the need for precipitating the poly(A)+ transcripts in the eluent. Intact poly(A)+ RNA which is fully representative of the mRNA population of the original sample can be obtained in less than one hour.
Note that for input amounts >5µg we recommend the NEBNext High Input Poly(A) mRNA Isolation Module (NEB #E3370).
- This product is related to the following categories:
- RNA Depletion & mRNA Enrichment,
- RNA Library Prep for Illumina,
- Next Generation Sequencing Library Preparation
- This product can be used in the following applications:
- RNA-seq,
- RNA Modification,
- RNA Purification and Isolation