Description:
TheNEBNext®Ultra™LigationModuleisdesignedforusewiththeNEBNextSingleplexorMultiplexOligosforIllumina.
TheNEBNextUltra™LigationModuleisDesignedforUsewiththeFollowingProducts:
NEBNextUltra™EndRepair/dA-TailingModule(NEB#E7442)
NEBNextSingleplexorMultiplexOligosforIllumina®(NEB#E7350,#E7335, #E7500,#E7710,#E7730,#E6609,#E7600)
NEBNextQ5HotStartHiFiPCRMasterMix(NEB#M0543)
NEBNextHigh-Fidelity2XPCRMasterMix(NEB#M0541)FunctionalValidation
EachsetofreagentsisfunctionallyvalidatedtogetherthroughconstructionandsequencingofatranscriptomelibraryandsequencedonaDNAlibraryontheIlluminasequencingplatform.
LotControl
Thelotsprovidedaremanagedseparatelyandqualifiedbyadditionalfunctionalvalidation.Individualreagentsundergostandardenzymeactivityandqualitycontrolassays,andalsomeetstringentcriteriaintheadditionalqualitycontrolslistedoneachindividualcomponentpage
ReagentsSupplied
Thefollowingreagentsaresuppliedwiththisproduct:
| Storeat(°C) | Concentration |
NEBNext®LigationEnhancer | -20 | |
Blunt/TALigaseMasterMix | -20 | |