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NEB/NEBNext® Ultra™ DNA Library Prep Kit for Illumina®/96 reactions/E7370L
  • NEB/NEBNext® Ultra™ DNA Library Prep Kit for Illumina®/96 reactions/E7370L

NEB/NEBNext® Ultra™ DNA Library Prep Kit for Illumina®/96 reactions/E7370L

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货号: E7370L
品牌: NEB
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    • The NEBNext® Ultra™ DNA Library Prep Kit for Illumina® contains reagents for preparation of libraries for next-generation sequencing on the Illumina platform from 5 ng – 1 µg input DNA, in a streamlined workflow. Please note that adaptors and primers are not included in the kit and are available separately.Each kit component must pass rigorous quality control standards, and each set of reagents is functionally validated together by construction and sequencing of a library on the Illumina sequencing platform.

      Ultra DNA Library Preparation Workflow for IlluminaUltra DNA Library Preparation Workflow for Illumina
      NEBNext Ultra™ DNA provides high library yields even with lowinputs and difficult samplesNEBNext Ultra™ DNA provides high library yields even with low inputs and difficult samples
      All libraries were prepared with 5 ng of input DNA and run on an Agilent Bioanalyzer®.A. E. coli genomic DNA libraries. 1: Ladder; 2: Library prepared using NEBNext DNALibrary Prep Master Mix Set (E6040); 3: Library prepared using NEBNext Ultra DNALibrary Prep Kit (E7370). With 5 ng inputs, library yields are significantly higher usingthe NEBNext Ultra™ DNA Kit.B. FFPE sample. Human lung tumor genomic DNA from a 66-year old male, extracted froma 10-year old FFPE block (Biochain, Inc.). 1: Library prepared using NEBNext Ultra DNALibrary Prep Kit (E7370). 2: Input DNA (highly degraded FFPE genomic DNA).
      Read depth correlation shows consistent high coverage for 5 ng - 1 µg input amountsRead depth correlation shows consistent high coverage for 5 ng - 1 µg input amounts
      Libraries were prepared with various amounts of human IMR90 gDNA (5, 50, and 1000 ng) and PCR amplified. A PCR-free library was also prepared with 1000 ng human IMR90 gDNA. Sequence coverage of 10 kb windows of the human genome was analyzed on Galaxy, and Pearson correlation coefficient (R2 values) were calculated using Microsoft® Excel®. All libraries are highly correlated with each other, independent of DNA input as well as PCR amplification. High coverage was achieved for all DNA input amounts.
      NEBNext Ultra DNA Kits display strong performance with input amounts as low as 5 ngNEBNext Ultra DNA Kits display strong performance with input amounts as low as 5 ng
      Input DNA: IMR90 genomic DNA (human fibroblast).PF Mismatch Rate: The rate of bases mismatching the reference for all bases aligned to the reference sequence.Percent Duplication: The percentage of mapped sequence that is marked as duplicate.Percent Chimeras: The percentage of reads that map outside of a maximum insert size or that have the two ends mapping to different chromosomes.This data illustrates the strong performance of the NEBNext Ultra DNA Library Prep Kit for Illumina, even with 5 ng of human genomic DNA input. Mismatch rate, % duplication and % chimeras are all low, and similar, regardless of input amount.

      Lot Control

      The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page
      This product is related to the following categories:
      Next Generation Sequencing Library Preparation
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