NEBNext Multiplex Oligos for Enzymatic Methyl-seq (Unique Dual Index Primer Pairs) is compatible with Illumina® sequencing and includes the NEBNext EM-seq™ Adaptor, and NEBNext PCR primers that enable incorporation of unique index pairs during library amplification.The 96-reaction size includes a single-use 96-well plate with a pierceable foil seal. Each well of the plate contains a unique pair of one i5 index primer and one i7 index primer, therefore providing 96 unique dual indices. For the 24-reaction size, indices are provided in 24 separate vials, with each vial containing a unique pair of one i5 index primer and one i7 index primer. There is no overlap between i5 and i7 index pairs provided in the 24 and 96 reaction kits.Although optimized for the EM-seq workflow, the NEBNext EM-seq Adaptor is also compatible with bisulfite sequencing workflows.These components are also provided in the NEBNext Enzymatic Methyl-seq Kit (NEB #E7120).
This product is related to the following categories:
NEBNext® Multiplex Oligos (Adaptors & Primers),
Epigenetic Analysis,
Epigenetics Products,
Next Generation Sequencing Library Preparation
This product can be used in the following applications: