The NEBNext Enzymatic Methyl-seq Conversion Module provides a high-performance enzyme-based alternative to bisulfite conversion for methylome analysis.In the EM-seq workflow, TET2 oxidizes 5-mC and 5-hmC, providing protection from deamination by APOBEC in the next step. In contrast, unmodified cytosines are deaminated to uracils.Features:
High efficiency conversion
Minimal DNA damage
Minimized GC bias
5-mC and 5-hmC detection
Complete kit including library prep reagents also available (NEB #E7120)
This product is related to the following categories:
Automation for NEBNext® NGS Library Prep,
Epigenetic Analysis,
Epigenetics Products
This product can be used in the following applications: