Incorporating a novel hairpin loop structure, the NEBNext Adaptor ligates with increased efficiency to end-repaired, dA-tailed DNA. The loop contains a U, which is removed by treatment with USER® Enzyme (a mix of UDG and Endo VIII), to open up the loop and make it available as a substrate for PCR. During PCR, barcodes can be incorporated by use of the NEBNext index primers, thereby enabling multiplexing. The 96 8-base index primers included in this kit are pre-mixed with the universal primer and are packaged in a single-use 96-well plate with a pierceable foil seal. NEBNext Oligos can be used with NEBNext products, and with other standard Illumina-compatible library preparation protocols. Additional single- or dual-barcode primer options are available, in 12- and 96- index formats (NEB #E7335, NEB #E7500, NEB #E7710, NEB #E7730, NEB #E7600). A methylated version of the NEBNext Adaptor is also available for use with bisulfite sequencing protocols (NEB #E7535).Figure 1: Workflow demonstrating the use of NEBNext Multiplex Oligos for Illumina (96 Index Primers)
This product is related to the following categories:
NEBNext® Multiplex Oligos (Adaptors & Primers),
Next Generation Sequencing Library Preparation
This product can be used in the following applications:
Applications of USER® and Thermolabile USER II Enzymes
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