The NEBNext® Single Cell/Low Input cDNA Synthesis & Amplification Module uses a template switching method to generate full length cDNAs directly from single cells or 2 pg – 200 ng RNA. This unique workflow enables generation of high quality sequencing data from a broad range of inputs, and superior transcript detection, while providing reliably consistent performance.
Features:
The highest yields from single cells, or 2 pg - 200 ng RNA
Input can be cultured or primary cells, or total RNA
Single-tube protocol from cell lysis to cDNA
Fast, streamlined, automation-friendly workflow, with minimal handling steps and hands-on time
Download extensive performance data in our technical note.