The NEBNext® Single Cell/Low Input cDNA Synthesis & Amplification Module uses a template switching method to generate full length cDNAs directly from single cells or 2 pg – 200 ng RNA. This unique workflow enables generation of high quality sequencing data from a broad range of inputs, and superior transcript detection, while providing reliably consistent performance.
Features:
The highest yields from single cells, or 2 pg - 200 ng RNA
Input can be cultured or primary cells, or total RNA
Single-tube protocol from cell lysis to cDNA
Fast, streamlined, automation-friendly workflow, with minimal handling steps and hands-on time
Download extensive performance data in our technical note.
Figure 1: NEBNext Single Cell/Low Input workflow
Figure 2: Higher cDNA yields with the NEBNext Single Cell/ Low Input cDNA Synthesis & Amplification Module
Sequencing libraries were generated from HeLa, Jurkat and M1 single cells, or 10 pg of Universal Human Reference (UHR) RNA (Agilent® #740000) with recommended amounts of ERCC RNA Spike-In Mix I (Thermo Fisher Scientific® #4456740). The NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module or the SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (Clontech® # 634891) were used. cDNA libraries were amplified using the number of PCR cycles shown. Error bars indicate standard deviation for 8-21 replicates.
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