Description:

TheNEBNext®MagnesiumRNAFragmentationModulehasbeenoptimizedtofragmentRNAintosmallpiecesusingdivalentcationsunderelevatedtemperature.
TheNEBNextMagnesiumRNAFragmentationModulecontainsbuffersthataresuitedforRNAfragmentation.Eachofthesecomponentsmustpassrigorousqualitycontrollistedoneachindividualcomponentpage.
Forlargervolumerequirements,customizedandbulkpackagingisavailablebypurchasingthroughtheOEM/BulksdepartmentatNEB.Pleasecontactnebsolutions@neb.comforfurtherinformation.
Figure1:RelativesizedistributionofeukaryoticmRNAfragmentsasseenusingtheBioanalyzer2100.
Poly(A)+mRNA(40ng)purifiedfromtheUniversalHumanReferenceTotalRNA(Stratagene)wasfragmentedin1XNEBNextMagnesiumRNAFragmentationBufferfor1–5minutesat94°C.FragmentationReactionwasstoppedin1XNEBNextFragmentationStopSolution.Sampleswerediluted1:10inNuclease-FreeWaterandanalyzedintheBioanalyzer2100.FunctionalValidation
Eachsetofreagentsisfunctionallyvalidatedtogetherthroughconstructionandsequencingofatranscriptomelibraryandsequencedona.
LotControl
Thelotsprovidedaremanagedseparatelyandqualifiedbyadditionalfunctionalvalidation.Individualreagentsundergostandardenzymeactivityandqualitycontrolassays,andalsomeetstringentcriteriaintheadditionalqualitycontrolslistedoneachindividualcomponentpage
ReagentsSupplied
Thefollowingreagentsaresuppliedwiththisproduct:
| Storeat(°C) | Concentration |
NEBNext®RNAFragmentationBuffer | -20 | |
NEBNextRNAFragmentationStopSolution | -20 | |