The NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module has been optimized to generate double-stranded cDNA from first-strand cDNA, as part of the NEBNext non-directional RNA library preparation workflow. The NEBNExt Ultra II Directional RNA Second Strand Synthesis Module is Designed for Use with the Following:
NEBNext Ultra II RNA First Strand Synthesis Module (NEB #E7771) or NEBNext RNA First Strand Synthesis Module (NEB #E7525)
NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546)or NEBNext Ultra End Repair/dA-Tailing Module (NEB #E7442)
NEBNext Ultra II Ligation Module (NEB #E7595) or NEBNext Ultra Ligation Module (NEB #E7445)
NEBNext Ultra™ II Q5® Master Mix (NEB #M0544) or NEBNext High Fidelity 2X PCR Master Mix (NEB #M0541)