Biotinazideisaderivativeofbiotin(vitaminH)covalentlyboundwithazidegroup.Thisreagentallowslabelingofvariousalkynylatedmolecules(suchasDNA,oligonucleotides,andproteins)withbiotin.Biotinbindingtoavidinorstreptavidincanbeusedindownstreamaffinityapplicationsfortheisolationofbiotinylatedmoleculesortheirbindingwithstreptavidinconjugates.
Thisstructureofbiotinazidefeatureslonghydrophiliclinkerwhichseparatesbiotinresiduefromthetargetmoleculeforefficientbindingwithstreptavidin.Itslinker(PEG)alsoenhancesaquoussolubilitytofacilitateconjugation.
Theazidecanbeconjugatedwithvariousmoleculesusingourrecommendedprotocol.
Recommendedprotocol
Click-ChemistryLabelingofOligonucleotidesandDNA
Generalproperties
Appearance: | colorlesssolid |
Molecularweight: | 400.50 |
Molecularformula: | C16H28N6O4S |
Solubility: | solubleinDMF,DMSO,moderatelysolubleinwater |
Qualitycontrol: | NMR1H(95%)and13C,TLC,functionaltesting |
Storageconditions: | Storage:24monthsafterreceivalat-20°Cinthedark.Transportation:atroomtemperatureforupto3weeks. |