当前位置 ： NEB >>> NEB/NEBNext® Microbiome DNA Enrichment Kit/E2612S/6 reactions
NEB/NEBNext® Microbiome DNA Enrichment Kit/E2612S/6 reactions
  • NEB/NEBNext® Microbiome DNA Enrichment Kit/E2612S/6 reactions

NEB/NEBNext® Microbiome DNA Enrichment Kit/E2612S/6 reactions

价格: ¥2520.00 市场价: 4200.00
打印产品信息
货号: E2612S-6reactions
品牌: NEB
规格
数量
库存(0)
特别 提示
代购产品：无质量问题不接受退换货，下单前请仔细核对信息。
下单后请及时联系客服核对商品价格，订单生效后再付款。
立即购买 加入购物车

收藏商品
资深产品顾问
咨询顾问

全国免费服务热线

4000-520-616

  • 自营商城 一站式服务
  • 厂家直采 剔除溢价
  • 品质甄选 正品保证
  • 严控流程 只做188精品
  • 极速物流 如约送货
  • 详情
  • 使用说明
  • 常见问题

    • Description:











      TheNEBNextMicrobiomeDNAEnrichmentKitfacilitatesenrichmentofmicrobialDNAfromsamplescontainingmethylatedhostDNA(includinghuman),byselectivebindingandremovaloftheCpG-methylatedhostDNA.Importantly,microbialdiversityremainsintactafterenrichment.

      InhumanDNA,4–6%ofcytosinesaremethylated,and60–90%ofthesemethylatedcytosinesareatCpGsites(1,2).Incontrast,methylationatCpGsitesinmicrobialspeciesisrare.TheNEBNextMicrobiomeDNAEnrichmentKitusesasimpleandfastmagneticbead-basedmethodtoselectivelybindandremoveCpG-methylatedhostDNA.ThemethodusestheMBD2-Fcprotein,whichiscomposedofthemethylatedCpG-specificbindingproteinMBD2,fusedtotheFcfragmentofhumanIgG.TheFcfragmentbindsreADIlytoProteinA,enablingeffectiveattachmenttoProteinA-boundmagneticbeads.TheMBD2domainofthisproteinbindsspecificallyandtightlytoCpGmethylatedDNA.ApplicationofamagneticfieldthenpullsouttheCpG-methylated(eukaryotic)DNA,leavingthenon-CpG-methylated(microbial)DNAinthesupernatant(3).Ifdesired,thehostDNAcapturedinthemagneticbeadpelletcanbeeluted,andaprotocolisprovidedforthis.

      TheNEBNextMicrobiomeDNAEnrichmentKitissuitableforawiderangeofsampletypes,includingsampleswithhighlevelsofcontaminatinghostDNA,(3,4)andiscompatIBLewithdownstreamapplicationsincludingnextgenerationsequencingonallplatforms,qPCRandend-pointPCR.

      MethodOverview:

      StepI—PrepareGenomicDNA
      DNAshouldbefreeofproteins,proteinaseA,SDSandorganicsolvents;sizeshouldbe≥15kbforoptimalperformance.

      StepII—CombineMBD2-FcandMagneticBeadsin1XBind/washBuffer.
      Incubatethereactionfor10minutesatroomtemperature.WashbeadstwotimesinBind/washReactionBuffer.

      StepIII—AddDNAtoMBD2-FcMagneticBeads.
      Incubatethereactionfor15minutesatroomtemperaturewithgentlemixing.

      StepIV—CollectSupernatantFractioncontainingenrichedmicrobialDNAandBeadFraction-ContainingHostDNA. 

      Figure1.Separationworkflow
      Figure 1. Separation workflow

      SalivaryMicrobiomeDNAEnrichment
      Salivary Microbiome DNA Enrichment
      DNAwaspurifiedfrompooledhumansalivaDNA(InnovativeResearch)andenrichedusingtheNEBNextMicrobiomeDNAEnrichmentKit.LibrarieswerepreparedfromunenrichedandenrichedsamplesandsequencedontheSOLiD4platform.Thegraphshowspercentagesof500M-537MSOLiD450bpreadsthatmappedtoeithertheHumanreferencesequence(hg19)ortoamicrobelistedinHumanOralMicrobiomeDatabase(HOMD)[1].(BecausetheHOMDcollectionisnotcomprehensive,~80%ofreadsintheenrichedsamplesdonotmaptoeitherdatabase.)ReadsweremappedusingBowtie0.12.7[2]withtypicalsettings(2mismatchesina28bpseedregion,etc.).
      MicrobiomeDiversityisRetainedafterEnrichmentwiththeNEBNextMicrobiomeDNAEnrichmentKit
      Microbiome Diversity is Retained after Enrichment with the NEBNext Microbiome DNA Enrichment Kit
      DNAwaspurifiedfrompooledhumansalivaDNA(InnovativeResearch)andenrichedusingtheNEBNextMicrobiomeDNAEnrichmentKit.Librarieswerepreparedfromunenrichedandenrichedsamples,followedbysequencingontheSOLiD4platform.ThegraphshowsacomparisonbetweenrelativeabundanceofeachbacterialspecieslistedinHOMD[1]beforeandafterenrichmentwiththeNEBNextMicrobiomeDNAEnrichmentKit.AbundanceisinferredfromthenumberofreadsmappingtoeachspeciesasapercentageofallreadsmappingtoHOMD.Highconcordancecontinueseventoverylowabundancespecies(inset).Wecompared501M50bpSOliD4readsintheenricheddatasetto537M50bpSOLiD4readsintheunenricheddataset.ReadsweremappedusingBowtie0.12.7[2]withtypicalsettings(2mismatchesina28bpseedregion,etc).
      *Niesseriaflavescens–Thisorganismmayhaveunusualmethylationdensity,allowingittobindtheenrichingbeadsatalowlevel.OtherNiesseriaspecies(N.mucosa,N.siccaandN.elognata)arerepresented,butdonotexhibitthisanomalousenrichment.

       
      EachkitcontainssufficientreagentsfortheeffectiveseparationofCpGmethylatedDNAfromamixedpoolcontainingmicrobialorviralDNA.Ifstartingwith1μgofinputDNAperexperiment,thevolumesprovidedaresufficientforpreparationsofupto6reactions(NEB#E2612S)and24reactions(NEB#E2612L).Allreactionsshouldbestoredat-20°.

      Box1:Storeat-20°C.
      Box2:Storeat4°C.Donotfreeze.

      ReagentsSupplied

      Thefollowingreagentsaresuppliedwiththisproduct:

      Storeat(°C)Concentration
      NEBNextMBD2-FcProtein-20
      NEBNextBind/washBuffer-205X
      16srRNAUniversalGeneBacteriaControlPrimers-20
      NEBNextProteinAMagneticBeads4
      RPL30HumanDNAControlPrimers-20
    售后保障
    蚂蚁淘生物188，秉承蚂蚁淘一贯的严谨态度，由蚂蚁淘公司专业人员负责品控、采购、物流、销售、售后，保障正品优质。以“快速好省，为科研提供好产品、好价格”为理念，直接链接原厂家，从全国各地原制造商严格挑选188款科研精品，剔除品牌溢价，188生物新电商，把好的产品带给科研！  力求给你最优质的商品。
  • Q：生物188产品正品保障吗？
    A：生物188质量把控人员具有十年的从业经验，在业界享有良好的口碑；自营商城，直接从厂家采购， 自己的团队负责国际物流和清关，中间没有第三方，所有流程严格把控，100%保证正品，假一罚十。

    Q：下单后可以修改订单吗？
    A：下单后的商品付款之前可以修改；订单付款成功，需要联系我们客服进行修改；客服电话：4000-520-616

    Q：可以开发票吗？
    A：本网站所售商品都是正规清关，均开具16%正规发票，发票金额含配送费金额，另有说明的除外。

    Q：商品几天可以发货？
    A：生物188商品，全部现货销售，付款后即可发货,一般一周内送达！

    Q：如何联系商家？
    A：有任何问题够可以电话咨询我们，全国24小时免费服务热线：4000-520-616 或联系我们的在线客服QQ：1570468124

    Q：收到的商品少了/发错了怎么办？
    A：同个订单购买多个商品可能会分为一个以上包裹发出，可能不会同时送达，建议查看订单详情是否是 部分发货状态；如未收到，可联系在线客服或者致电4000-520-616。

    Q：退换货/维修需要多长时间？
    A：一般情况下，退货处理周期为客户收到产品一个月内（以快递公司显示签收时间为准），包装规格、 数量、品种不符，外观毁损、短缺或缺陷，请在收到货24小时内申请退换货；特殊商品以合同条款为准。

客户服务

在线客服
用户反馈

何为188

极简而严谨，我们仅销售188款生物医学科研用品，款款都是爆款；因为少所以聚焦，聚焦甄选每一款产品，聚焦服务每一位客户!

关注我们 :

点击QQ联系我们
生物188微信

关注188微信公众号

获取最新优惠活动通知

  • 品质甄选，正品保证

  • 自营电商，厂家直采

  • 极简主义，188精品

常见问题 | 购买相关 | 配送与验收 | 支付问题 | 售后与服务 | 服务协议 | 隐私政策 | 何为188 | 中国智造 | 联系我们
友情链接： AffinityImmuno中国 | Polysciences第二代聚合物转染 | Immune Biosolutions公 | leica819 | AsisChem产品代理 | dextra厂家 | Lumigen Inc代理 | 蚂蚁淘 | 广州麦煌机械设备有限公司

公司版权所有 © 2005-2021 苏州蚂蚁淘生物科技有限公司 苏ICP备17049038号-10