Description:
TheWarmStartLAMPKit(DNA&RNA)isdesignedtoprovideasimple,one-stepsolutionforLoop-MediatedIsothermalAmplification(LAMP)ofDNAorRNA(RT-LAMP)targets.LAMPandRT-LAMParecommonlyusedisothermalamplificationtechniquesthatprovidesrapiddetectionofatargetnucleicacidusingLAMP-specificprimers(suppliedbytheuser)andastrand-displacingDNApolymerase.ThiskitissuppliedwiththeWarmStartLAMP2XMasterMix,whichcontainsablendofBst2.0WarmStartDNAPolymeraseandWarmStartRTxReverseTranscriptaseinanoptimizedLAMPbuffersolution.BothBst2.0WarmStartDNAPolymeraseandWarmStartRTxReverseTranscriptasehavebeenengineeredforimprovedperformanceinLAMPandRT-LAMPreactions.Afluorescentdyeisalsosuppliedtoenablereal-timefluorescencemeasurementofLAMP.TheWarmStartLAMPKitiscompatIBLewithmultipledetectionmethods,includingturbiditydetection,real-timefluorescencedetection(whenusedwithLAMPfluorescentdye)andend-pointvisualization.
NEB’sWarmStartLAMPKit(DNA&RNA)iscompatiblewithmultipledetectionmethods
NEB’sWarmStartLAMPKit(DNA&RNA)offersspeedandrobustsensitivity
NEB’sWarmStartLAMPKit(DNA&RNA)providesfastLAMP/RT-LAMPresults
NEBWarmStartLAMPKit(DNA&RNA)enablesspecificamplificationacrossmultiplespecies/targets
PRIMERSET | DNATEMPLATE |
---|
HeLa DNA | Lyme DNA | B.malayi DNA | E.coli DNA | C.elegans DNA |
---|
BRCAb(human) | + | | | | |
CFTR(human) | + | | | | |
LymO(Lyme) | | + | | | |
BmHhal(B.malayi) | | | + | | |
dnaE(E.coli) | | | | + | |
Lec10b(C.elegans) | | | | | + |
Lec6(C.elegans) | | | | | + |
Ineachindividualreaction,multiplegDNAtemplateswereaddedalongwithprimersspecifictoatargetinonlyonespecies.Reactionswereincubatedat65°Cfor74minutesonathermocycler(Bio-RadCFX96)intriplicate.Positiveresults(+)wereobservedonlyintheexpectedspecies/targetcombinations,demonstratinghighspecificityoftheWarmStartLAMPreactions.
KitComponents
Thefollowingreagentsaresuppliedwiththisproduct:
| Storeat(°C) | Concentration |
WarmStartLAMP2XMasterMix | -20 | |
LAMPFluorescentDye(50X) | -20 | |