NEB offers the Quick Blunting™ and Quick Ligation™ Kits together as a bundle at a value price.The Quick Blunting™ Kit is used to convert DNA with incompatible 5´or 3´overhangs to 5´phosphorylated, blunt-ended DNA for efficient blunt-end ligation into DNA cloning vectors. DNA is blunted using T4 DNA polymerase (NEB #M0203) which has both 3´→5´ exonuclease activity and 5´→ 3´ polymerase activity. T4Polynucleotide Kinase (NEB #M0201) is included in the enzyme mix for phosphorylation of the 5´ends of blunt-ended DNA for subsequent ligation into a cloning vector. This kit is optimized for blunting up to 5 µg of DNA in a single reaction. The Quick Ligation™ Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature (25°C).
This product is related to the following categories:
DNA Ligases Products
This product can be used in the following applications:
Blunting,
Phosphorylation (Kinase),
Cloning Ligation,
Fast Cloning: Accelerate your cloning workflows with reagents from NEB
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