Echelon/PTEN Enzyme, active/10 ug/E-3000

PTEN Enzyme, active is a N-Terminal GST-Tagged, recombinant human PTEN (Phosphatase and Tensin Homolog), 76kD, purified from E.coli using glutathione-sepharose column chromatography.

Storage: Store product > -70 ºC. Enzyme is stable for at least 6 months at -70 ºC as undiluted stockSpecificity: PTEN selectively removes phosphate from the 3’ position of the inositol ring of PtdIns(3,4,5)P₃Specific Activity: request Certificate of Analysis (COA)

The PTEN enzyme is a 3-phosphoinositide phosphatase that converts PI(3,4,5)P₃ to PI(4,5)P₂; thus opposing PKB/Akt activation by PI3-K. PTEN is involved in neuronal stem cell proliferation and self-renewal, cardiac myocyte hypertrophy and contractility, and a wide range of developmental processes. PTEN, however, is best known for its role as a tumor suppressor. Loss of PTEN activity results in accumulation of PI(3,4,5)P₃, abnormal activation of PKB/Akt, unregulated cell growth, suppression of apoptosis, and increase tumorigenesis in a number of human tissues. It has also been proposed that PTEN is a candidate for targeted chemotherapy because certain anti-cancer agents preferentially destroy tumors with PTEN mutations. In addition to this direct role in cancer, PTEN also indirectly regulates cancer-associated pathways including VEGF-mediated angiogenesis among others.

Publications

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1. Brooks, R., et al. (2010). “SHIP1 inhibition increases immunoregulatory capacity and triggers apoptosis of hematopoietic cancer cells.” J Immunol 184(7): 3582-3589.2. Caromile, L. A., et al. (2010). “The neurosecretory vesicle protein phogrin functions as a phosphatidylinositol phosphatase to regulate insulin secretion.” J Biol Chem 285(14): 10487-10496.3. Thakral, C., et al. (2012). “ALK+ anaplastic large cell lymphoma exhibits phosphatidylinositol-3 kinase/akt activity with retained but inactivated PTEN—A report from the Children’s Oncology Group.” Pediatric Blood & Cancer 59(3): 440-447.4. Steidle, E. A., et al. (2016). “A novel inositol pyrophosphate phosphatase in Saccharomyces cerevisiae: Siw14 selectively cleaves the b-phosphate from 5-diphosphoinositol pentakisphosphate (5PP-IP5).” J. Biol. Chem. 291: 6772-6783.5. Alsina-Sanchís, E., et al. (2018). “ALK1 Loss Results in Vascular Hyperplasia in Mice and Humans Through PI3K Activation.” Arteriosclerosis, thrombosis, and vascular biology 38(5): 1216-1229.