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Abnova/CDKN1A(phospho T145) & CDKN1A Protein Phosphorylation Antibody Pair/1kit/DP0202
  • Abnova/CDKN1A(phospho T145) & CDKN1A Protein Phosphorylation Antibody Pair/1kit/DP0202

Abnova/CDKN1A(phospho T145) & CDKN1A Protein Phosphorylation Antibody Pair/1kit/DP0202

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货号: DP0202
品牌: Abnova
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  • 使用说明
  • 常见问题
      • Specification
      • Gene Info
      • Interactome
      • Pathway
      • Disease
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      • Specification
      • Product Description:
      • This protein phosphorylation antibody pair set comes with two antibodies, one against the CDKN1A protein, and the other against the specific T145 phosphorylated site of CDKN1A for use in in situ Proximity Ligation Assay. See Publication Reference below.DuoLink
      • Reactivity:
      • Human
      • Quality Control Testing:
      • Dual recognition immunofluorescence result.QC Testing of DP0202Representative image of Proximity Ligation Assay of protein phosphorylation. HeLa cells were stained with dual recognition antibody pair set, rabbit polyclonal antibody 1:1200 and mouse monoclonal antibody 1:50. Each red dot represents one single phosphorylated protein. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
      • Supplied Product:
      • Antibody pair set content: 1. Phospho-CDKN1A T145 rabbit polyclonal antibody (20 ul) In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (0.02% sodium azide, 50% glycerol) 2. CDKN1A mouse monoclonal antibody (40 ug) In 1x PBS, pH 7.2 *Reagents are sufficient for at least 30-50 assays using recommended protocols.
      • Storage Instruction:
      • Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
      • Datasheet:
      • PDF DownloadDownload
      • Publication Reference
      • 1.
      • In situ detection of phosphorylated platelet-derived growth factor receptor beta using a generalized proximity ligation method.Jarvius M, Paulsson J, Weibrecht I, Leuchowius KJ, Andersson AC, Wahlby C, Gullberg M,Botling J,Sjoblom T, Markova B, Ostman A, Landegren U, Soderberg O.Mol Cell Proteomics. 2007 Sep;6(9):1500-9. Epub 2007 Jun 12.
      • 2.
      • Direct observation of individual endogenous protein complexes in situ by proximity ligation.Soderberg O, Gullberg M, Jarvius M , Ridderstrale K, Leuchowius KJ, Jarvius J, Wester K, Hydbring P, Bahram F, Larsson LG, and Landegren U. Nat Methods. 2006 Dec;3(12):995-1000. Epub 2006 Oct 29.
      • 3.
      • Cytokine detection by antibody-based proximity ligation.Gullberg M, Gustafsdottir SM, Schallmeiner E, Jarvius J, Bjarnegard M, Betsholtz C, Landegren U, andFredriksson S. Proc Natl Acad Sci U S A. 2004 Jun 1;101(22):8420-4. Epub 2004 May 21.
      • 4.
      • Protein detection using proximity-dependent DNA ligation assays.Fredriksson S, GullbergM, Jarvius J, Olsson C, Pietras K, Gustafsdottir SM, Ostman A, and Landegren U. Nat Biotechnol. 2002 May;20(5):473-7.
      • 5.
      • Highly specific detection of phosphorylated proteins by DuolinkMats Gullberg and Ann-Catrin Andersson Nature Methods 6. 2009
      • Applications
      • In situ Proximity Ligation Assay (Cell)
      • Application Image
      • In situ Proximity Ligation Assay (Cell)
      • Gene Information
      • Entrez GeneID:
      • 1026
      • Gene Name:
      • CDKN1A
      • Gene Alias:
      • CAP20,CDKN1,CIP1,MDA-6,P21,SDI1,WAF1,p21CIP1
      • Gene Description:
      • cyclin-dependent kinase inhibitor 1A (p21, Cip1)
      • Omim ID:
      • 116899
      • Gene Ontology:
      • Hyperlink
      • Gene Summary:
      • This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported. [provided by RefSeq
      • Other Designations:
      • CDK-interaction protein 1,DNA synthesis inhibitor,OTTHUMP00000016298,cyclin-dependent kinase inhibitor 1A,melanoma differentiation associated protein 6,wild-type p53-activated fragment 1
      • Interactome
      • Interactome
      • Gene Pathway
      • Bladder cancer
      • Cell cycle
      • Chronic myeloid leukemia
      • ErbB signaling pathway
      • Glioma
      • Melanoma
      • p53 signaling pathway
      • Pathways in cancer
      • Prostate cancer
      • Related Disease
      • Adenocarcinoma
      • Ataxia telangiectasia
      • Atherosclerosis
      • Atherosclerosis
      • Bowen"s Disease
      • Brain Neoplasms
      • Breast cancer
      • Breast Neoplasms
      • Carcinoma
      • Carcinoma, Basal Cell
      • Carcinoma, Squamous Cell
      • Carcinoma, Transitional Cell
      • Cardiovascular Diseases
      • Chromosome Aberrations
      • Chronic Disease
      • Colorectal Neoplasms
      • Diabetes Mellitus, Type 2
      • Disease Progression
      • DNA Damage
          • ... see more
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