Capacity:forstimulationof1×108cells
Content:6nmol/peptideOverview
PepTivator®A.fumigatusGel1isapooloflyophilizedpeptides,consistingmainlyof15-mersequenceswith11aminoacidsoverlap,coveringthecompletesequenceoftheAspergillusfumigatusGel1protein(UniProtID:P0C7S9).
Invitrostimulationofantigen-specificTcellswithPepTivatorPeptidePoolscausesthesecretionofeffectorcytokinesandtheup-regulationofactivation
Markers,whichthenallowthedetectionandisolationofantigen-specificTcells.
Details
Backgroundinformation
A.fumigatusisacommonsaprophyticfungus.Itcancauseallergicbronchopulmonaryaspergillosisorcaninducesevereinfectionsoftherespiratorytractinimmunocompromisedindividuals.ItissuggestedthatCD4+Tcellsplayacentralroleinthepreventionandeliminationoftheseinfections.
Downstreamapplications
PepTivatorA.fumigatusGel1–researchgradehasbeenspecificallydevelopedforefficientinvitrostimulationofA.fumigatusGel1–specificTcells.
Peptidesof15aminoacidsinlengthand11aminoacidsoverlaprepresentanoptimizedsolutionforstimulatingbothCD4+andCD8+Tcellsinvariousapplications,including:
- DetectionandanalysisofGel1-specificeffector/memoryTcellsinPBMCsbyMACS®CytokineSecretionAssays,intracellularcytokinestaining,orothertechnologies.
- IsolationofviableGel1-specificCD4+TcellswiththeCD154MicroBeadKit,orofCD4+andCD8+TcellsusingtheCD137MicroBeadKitorMACSCytokineSecretionAssay–CellEnrichmentandDetectionKits.Subsequently,cellscanbeexpandedforgenerationofTcelllines.
- GenerationofGel1-specificeffector/memoryTcellsfromnaiveTcellpopulations.
- Pulsingofantigen-presentingcells.